Department of Chemical and Biochemical Engineering, College of Engineering, Dongguk University-Seoul, 30 Pildong-ro 1-gil, Jung-gu, Seoul, 04620, Republic of Korea.
Mikrochim Acta. 2024 Aug 5;191(9):507. doi: 10.1007/s00604-024-06578-9.
An electrochemical sensor based on an electroactive nanocomposite was designed for the first time consisting of electrochemically reduced graphene oxide (ERGO), polyaniline (PANI), and poly(alizarin red S) (PARS) for ciprofloxacin (CIPF) detection. The ERGO/PANI/PARS-modified screen-printed carbon electrode (SPCE) was constructed through a three-step electrochemical protocol and characterized using FTIR, UV-visible spectroscopy, FESEM, CV, LSV, and EIS. The new electrochemical CIPF sensor demonstrated a low detection limit of 0.0021 μM, a broad linear range of 0.01 to 69.8 μM, a high sensitivity of 5.09 μA/μM/cm, and reasonable selectivity and reproducibility. Moreover, the ERGO/PANI/PARS/SPCE was successfully utilized to determine CIPF in milk with good recoveries and relative standard deviation (< 5%), which were close to those with HPLC analysis.
首次设计了一种基于电活性纳米复合材料的电化学传感器，该传感器由电化学还原氧化石墨烯（ERGO）、聚苯胺（PANI）和聚（茜素红 S）（PARS）组成，用于环丙沙星（CIPF）检测。通过三步电化学协议构建了 ERGO/PANI/PARS 修饰的丝网印刷碳电极（SPCE），并通过 FTIR、UV-可见光谱、FESEM、CV、LSV 和 EIS 进行了表征。新型电化学 CIPF 传感器的检测限低至 0.0021 μM，线性范围宽达 0.01 至 69.8 μM，灵敏度高为 5.09 μA/μM/cm，具有合理的选择性和重现性。此外，ERGO/PANI/PARS/SPCE 成功用于牛奶中环丙沙星的测定，回收率和相对标准偏差（<5%）良好，与 HPLC 分析结果相近。
