从水提取物和碱性水相部分生物合成的银纳米聚集体对某些物种的抗真菌活性。（原文中“from the Aqueous Extract and the Alkaline Aqueous Fraction of ”以及“against Some Species”部分信息不完整）

The Antifungal Activities of Silver Nano-Aggregates Biosynthesized from the Aqueous Extract and the Alkaline Aqueous Fraction of against Some Species.

作者信息

Al-Otibi Fatimah

机构信息

Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.

出版信息

Nanomaterials (Basel). 2023 Dec 28;14(1):88. doi: 10.3390/nano14010088.

DOI:10.3390/nano14010088

PMID:38202544

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10780319/

Abstract

is a major medicinal species used in indigenous medicinal herbal medications in South Asia, the Middle East, Iran, and Iraq to treat a variety of ailments. The current study aimed to investigate the antifungal properties of biosynthesized silver nanoparticles (AgNPs) made from aqueous extract and its alkaline aqueous fraction. Fourier transform infrared spectroscopy (FTIR), UV-vis spectrophotometry, dynamic light scattering (DLS), and transmitted electron microscopy (TEM) were used to characterize AgNPs. The produced extracts and AgNPs were tested for their antifungal efficacy against four spp. All of the characterization experiments proved the biosynthesis of targeted AgNPs. FTIR showed a wide distribution of hydroxyl, amino, carboxyl, and alkyl functional groups among all preparations. The DLS results showed that the produced Aq-AgNPs and the Alk-AgNPs had an average size of 95.9 nm and 54.04 nm, respectively. On the other hand, TEM results showed that the Aq-AgNPs and Alk-AgNPs had average diameters ranging from 21 to 90 nm and 7.25 to 25.32 nm. Both AgNPs absorbed UV light on average at 405 nm and 415 nm, respectively. Regarding the fungicidal activity, the highest doses of Aq-extract and Aq-AgNPs inhibited the mycelial growth of (19.8%, 87.5%), (28.1%, 72.3%), (37.5%, 75%), and (27.1%, 62.5%), respectively ( < 0.001). Interestingly, the Alk-fraction had stronger inhibition than the biosynthesized AgNPs, which resulted in complete inhibition at the doses of 10% and 20% ( < 0.001). Furthermore, microscopic analysis demonstrated that both AgNPs caused obvious morphological alterations in the treated organisms when compared to the control. In conclusion, 's Aq-extract, alkaline fraction, and their biosynthesized AgNPs show substantial antifungal efficacy against several spp. It is the first study to highlight the prospective biological activities of Aq-extract and its alkaline fraction against , , and . In addition, it is the first opportunity to deeply investigate the ultrastructural changes induced in the species treated with crude Aq-extract and its biosynthesized AgNPs. More studies are required to investigate their biological effect against other or fungal species.

摘要

是南亚、中东、伊朗和伊拉克本土草药中用于治疗多种疾病的主要药用物种。本研究旨在探究由水提取物及其碱性水相馏分制备的生物合成银纳米颗粒（AgNPs）的抗真菌特性。采用傅里叶变换红外光谱（FTIR）、紫外可见分光光度法、动态光散射（DLS）和透射电子显微镜（TEM）对AgNPs进行表征。对所制备的提取物和AgNPs针对四种[物种名称未给出]的抗真菌功效进行了测试。所有表征实验均证实了目标AgNPs的生物合成。FTIR显示所有制剂中羟基、氨基、羧基和烷基官能团分布广泛。DLS结果表明，所制备的水相AgNPs（Aq - AgNPs）和碱性相AgNPs（Alk - AgNPs）的平均粒径分别为95.9 nm和54.04 nm。另一方面，TEM结果显示，Aq - AgNPs和Alk - AgNPs的平均直径分别为21至90 nm和7.25至25.32 nm。两种AgNPs平均分别在405 nm和415 nm处吸收紫外光。关于杀菌活性，水提取物（Aq - extract）和Aq - AgNPs的最高剂量分别抑制了[物种名称未给出]（19.8%，87.5%）、[物种名称未给出]（28.1%，72.3%）、[物种名称未给出]（37.5%，75%）和[物种名称未给出]（27.1%，62.5%）的菌丝生长（P < 0.001）。有趣的是，碱性馏分的抑制作用比生物合成的AgNPs更强，在10%和20%的剂量下导致完全抑制（P < 0.001）。此外，显微镜分析表明，与对照相比，两种AgNPs均使处理后的生物体出现明显的形态改变。总之，[物种名称未给出]的水提取物、碱性馏分及其生物合成的AgNPs对几种[物种名称未给出]显示出显著的抗真菌功效。这是首次强调[物种名称未给出]水提取物及其碱性馏分对[物种名称未给出]、[物种名称未给出]和[物种名称未给出]潜在生物活性的研究。此外，这是首次深入研究[物种名称未给出]粗水提取物及其生物合成的AgNPs处理的[物种名称未给出]物种中诱导的超微结构变化。需要更多研究来探究它们对其他[物种名称未给出]或真菌物种的生物学效应。