Al-Otibi Fatimah, Alfuzan Shahad A, Alharbi Raedah I, Al-Askar Abdulaziz A, Al-Otaibi Rana M, Al Subaie Hajar F, Moubayed Nadine M S
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Saudi J Biol Sci. 2022 Apr;29(4):2772-2781. doi: 10.1016/j.sjbs.2021.12.056. Epub 2022 Jan 3.
The green silver nanoparticles (green AgNPs) exhibit an exceptional antimicrobial property against different microbes, including bacteria and fungi. The current study aimed to compare the antifungal activities of both the crude aqueous extract of or different preparations of green AgNPs biosynthesized by mixing that aqueous extract with silver nitrate (AgNO). Two preparations of the green AgNPs were synthesized either by mixing the aqueous extract of with silver nitrate (AgNO) (normal AgNPs) or either irradiation of the AgNPs, previously prepared, under Co γ-ray using chitosan (gamma-irradiated AgNPs). Characterization of different AgNPs were tested by Zeta potential analyzer, Ultraviolet (UV) Visible Spectroscopy, and Fourier-Transform Infrared (FTIR) spectrometry. Three different plant pathogenic fungi were tested, , , and sp. The antifungal activities were evaluated by Transmission Electron Microscope (TEM) for either the crude aqueous extract of at three doses (25%, 50%, and 100%) or the newly biosynthesized AgNPs, normal or gamma-irradiated. With a few exceptions, the comparative analysis revealed that the irradiated green AgNPs at all three concentrations showed a relatively stronger antifungal effect than the normal AgNPs against all the three selected fungal strains. UV-visible spectroscopy of both preparations showed surface plasmon resonance at 421 nm. TEM results showed that both AgNPs were aggregated and characterized by a unique spherical shape, however, the gamma-irradiated AgNPs were smaller than the non-irradiated AgNPs (0.007-0.026 µM vs. 0.009-0.086 µM). TEM photographs of the fungal strains treated with the two AgNPs preparations showed flaccid structures, condensed hyphae, and shrunken surface compared with control cells. The data suggested that the biosynthesized AgNPs have antifungal properties against , , and sp. These AgNPs may be considered a fungicide to protect different plants against phytopathogenic fungi.
绿色银纳米颗粒（绿色AgNPs）对包括细菌和真菌在内的不同微生物具有卓越的抗菌性能。当前研究旨在比较[植物名称]的粗水提取物或通过将该水提取物与硝酸银（AgNO₃）混合生物合成的绿色AgNPs的不同制剂的抗真菌活性。绿色AgNPs的两种制剂分别通过将[植物名称]的水提取物与硝酸银（AgNO₃）混合（普通AgNPs）或使用壳聚糖对先前制备的AgNPs进行钴γ射线辐照（γ射线辐照AgNPs）来合成。通过zeta电位分析仪、紫外可见光谱和傅里叶变换红外光谱对不同的AgNPs进行表征测试。测试了三种不同的植物病原真菌，即[真菌名称1]、[真菌名称2]和[真菌名称3]。通过透射电子显微镜（TEM）评估粗水提取物在三种剂量（25%、50%和100%）下或新生物合成的普通或γ射线辐照的AgNPs的抗真菌活性。除少数例外，比较分析表明，在所有三种浓度下，辐照后的绿色AgNPs对所有三种选定的真菌菌株的抗真菌作用均比普通AgNPs相对更强。两种制剂的紫外可见光谱均显示在421nm处有表面等离子体共振。TEM结果表明，两种AgNPs均发生聚集且具有独特的球形特征，然而，γ射线辐照的AgNPs比未辐照的AgNPs更小（0.007 - 0.026µM对0.009 - 0.086µM）。用两种AgNPs制剂处理的真菌菌株的TEM照片显示，与对照细胞相比，其结构松弛、菌丝浓缩且表面收缩。数据表明，生物合成的[植物名称]AgNPs对[真菌名称1]、[真菌名称2]和[真菌名称3]具有抗真菌特性。这些AgNPs可被视为一种杀菌剂，用于保护不同植物免受植物病原真菌侵害。
