Savvidou Maria G, Kontari Evgenia, Kalantzi Styliani, Mamma Diomi
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 9 Iroon Polytechniou Str, 15780 Athens, Greece.
Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
Materials (Basel). 2023 Dec 29;17(1):187. doi: 10.3390/ma17010187.
The green synthesis of silver nanoparticles (AgNPs) using the cell-free supernatant of a culture (CFS) was implemented in the current study, under illumination conditions. The reduction of Ag to AgNPs by the CFS could be described by a pseudo-first-order kinetic equation at the temperature range tested. A high reaction rate during synthesis and stable AgNPs were obtained at 45 °C, while an alkaline pH (pH = 11.0) and a AgNO aqueous solution to CFS ratio of 90:10 (/) proved to be the most effective conditions in AgNPs synthesis. A metal precursor (AgNO) at the concentration range tested (1-5 mM) was the limited reactant in the synthesis process. The synthesis of AgNPs was accomplished under static and agitated conditions. Continuous stirring enhanced the rate of reaction but induced aggregation at prolonged incubation times. Zeta potential and polydispersity index measurements indicated stable AgNPs and the majority of AgNPs formation occurred in the monodisperse phase. The X-ray diffraction (XRD) pattern revealed the face-centered cubic structure of the formed AgNPs, while TEM analysis revealed that the AgNPs were of a quasi-spherical shape with a size from 30 to 50 nm. The long-term stability of the AgNPs could be achieved in darkness and at 4 °C. In addition, the synthesized nanoparticles showed antibacterial activity against .
本研究在光照条件下，利用培养物的无细胞上清液（CFS）实现了银纳米颗粒（AgNPs）的绿色合成。在测试的温度范围内，CFS将Ag还原为AgNPs的过程可用准一级动力学方程描述。在45°C时合成过程中反应速率高且AgNPs稳定，而碱性pH值（pH = 11.0）和AgNO水溶液与CFS的比例为90:10（/）被证明是合成AgNPs最有效的条件。在所测试的浓度范围（1 - 5 mM）内，金属前驱体（AgNO）是合成过程中的限量反应物。AgNPs的合成在静态和搅拌条件下完成。持续搅拌提高了反应速率，但在长时间孵育时会诱导聚集。zeta电位和多分散指数测量表明AgNPs稳定，且大多数AgNPs形成于单分散相。X射线衍射（XRD）图谱揭示了所形成的AgNPs的面心立方结构，而透射电子显微镜（TEM）分析表明AgNPs为准球形，尺寸在30至50 nm之间。AgNPs在黑暗和4°C条件下可实现长期稳定性。此外，合成的纳米颗粒对……显示出抗菌活性。
