登革病毒感染小鼠脾脏中T淋巴细胞的差异性耗竭。
Differential depletion of T lymphocytes in the spleen of dengue virus-infected mice.
作者信息
Tandon P, Chaturvedi U C, Mathur A
出版信息
Immunology. 1979 May;37(1):1-6.
Following the i.c. inoculation of dengue type 2 virus (DV) the spleen weight of infected mice was reduced, as was the proportion of cells killed by ATS and complement (T lymphocytes) in spleen-cell suspensions. In DV-infected mice the mean haemolysin titre, 16 days after i.p. inoculation of 4 x 10(8) SRBC, was 47 compared with 406 in normal mice and spleen cells from DV-infected mice produced significantly reduced direct GVH reactivity in Parker strain (PS) infant mice. Adoptive transfer of spleen cells obtained from mice given three weeks i.p. doses of DV or a single i.c. dose, suppressed antigen-specific antibody secretion as detected by Jerne plaque technique. This suppression was abrogated by pretreating the transferred cells with ATS and complement. Thus DV selectively depletes T-lymphocyte subpopulations responsible for helper and effector functions and spares suppressor T cells in the spleen of infected mice.
在脑内接种2型登革病毒（DV）后，感染小鼠的脾脏重量减轻，脾脏细胞悬液中被抗胸腺细胞血清（ATS）和补体杀死的细胞（T淋巴细胞）比例也降低。在感染DV的小鼠中，腹腔注射4×10⁸个绵羊红细胞（SRBC）16天后，平均溶血素滴度为47，而正常小鼠为406，且感染DV小鼠的脾细胞在帕克株（PS）幼鼠中产生的直接移植物抗宿主（GVH）反应显著降低。对腹腔注射三周剂量的DV或单次脑内注射的小鼠获取的脾细胞进行过继转移，如通过耶尔恩斑技术检测，可抑制抗原特异性抗体分泌。用ATS和补体预处理转移细胞可消除这种抑制作用。因此，DV选择性地耗尽了感染小鼠脾脏中负责辅助和效应功能的T淋巴细胞亚群，而保留了抑制性T细胞。
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