Li Xun, Liu Bide, Wang Shuheng, Dong Qiang, Li Jiuzhi
Department of Urology, People s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China.
Department of Urology, People s Hospital of Xinjiang Uygur Autonomous Region, No. 91, Tianchi Road, Tianshan District, Urumqi, Xinjiang Uygur Autonomous Region, China.
Open Med (Wars). 2024 Jan 4;19(1):20230875. doi: 10.1515/med-2023-0875. eCollection 2024.
Prostate cancer (PCa) represents a substantial global health concern and a prominent contributor to male cancer-related mortality. The aim of this study is to explore the role of B-type endothelin receptor (EDNRB) in PCa and evaluate its therapeutic potential. The investigation employed predictive methodologies encompassing data acquisition from the GEO and TCGA databases, gene screening, enrichment analysis, experiments involving PCR, Western blotting, wound healing, and Transwell assays, as well as animal experiments. Analysis revealed a significant downregulation of EDNRB expression in PCa cells. Overexpression of EDNRB demonstrated inhibitory effects on tumor cell growth, migration, and invasion, likely mediated through activation of the cGMP-Protein Kinase G pathway. experiments further confirmed the tumor-suppressive properties of EDNRB overexpression. These findings underscore the prospect of EDNRB as a therapeutic target for PCa, offering novel avenues for PCa treatment strategies.
前列腺癌（PCa）是一个重大的全球健康问题，也是男性癌症相关死亡率的主要促成因素。本研究的目的是探讨B型内皮素受体（EDNRB）在前列腺癌中的作用，并评估其治疗潜力。该研究采用了预测性方法，包括从GEO和TCGA数据库获取数据、基因筛选、富集分析、PCR、蛋白质免疫印迹、伤口愈合和Transwell实验以及动物实验。分析显示，EDNRB在前列腺癌细胞中的表达显著下调。EDNRB的过表达对肿瘤细胞的生长、迁移和侵袭具有抑制作用，这可能是通过激活cGMP-蛋白激酶G途径介导的。实验进一步证实了EDNRB过表达的肿瘤抑制特性。这些发现强调了EDNRB作为前列腺癌治疗靶点的前景，为前列腺癌治疗策略提供了新途径。
