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埃及葫芦品种的形态、生化和分子多样性评估。

Morphological, Biochemical, and Molecular Diversity Assessment of Egyptian Bottle Gourd Cultivars.

机构信息

Department of Cross-Pollinated Vegetable Research, Horticulture Research Institute, Agricultural Research Centre, Giza 12619, Egypt.

Biology Department, College of Science, Jouf University, Sakaka 2014, Aljouf, Saudi Arabia.

出版信息

Genet Res (Camb). 2024 Jan 3;2024:4182158. doi: 10.1155/2024/4182158. eCollection 2024.

DOI:10.1155/2024/4182158
PMID:38205231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10781529/
Abstract

The genetic variability and relationships between ten bottle gourd cultivars were evaluated based on morphological, biochemical, and molecular parameters. The results displayed high variability among selected cultivars in terms of photosynthetic pigments, total free amino acids, total phenol content, isozymes pattern, and protein electrophoresis. Furthermore, differences in molecular markers were revealed by the SCoT technique. The peroxidase (POD) and polyphenyl oxidase (PPO) isozymes patterns did not detect significant differences in bands among cultivars. The protein patterns revealed seventeen bands ranging from 126 to 9 kDa and five polymorphic bands representing 29.41%. On the other hand, eight SCoT primers were used to evaluate the genetic variability and relationships between the ten Egyptian bottle gourd cultivars. The results of SCoT analysis detected 44 amplicons with 50% polymorphism. In addition, the results of the phylogenetic tree that is constructed based on the similarity coefficient revealed by SCoT analysis confirm the results of biochemical analysis indicating a genetic relationship between the most efficient bottle gourd cultivars (S1 and S2 cultivars). In addition, there is a genetic relationship among the less efficient bottle gourd cultivars (S4 and S5 cultivars). These results could be beneficial to distinguish among bottle gourd cultivars in the plant breeding programs.

摘要

基于形态学、生化和分子参数，评估了 10 个葫芦品种的遗传变异和关系。结果显示，所选品种在光合色素、总游离氨基酸、总酚含量、同工酶模式和蛋白质电泳方面表现出高度的变异性。此外，SCoT 技术揭示了分子标记的差异。过氧化物酶（POD）和多酚氧化酶（PPO）同工酶模式在品种间没有检测到带的显著差异。蛋白质图谱显示，从 126 到 9 kDa 之间有 17 条带，其中 5 条多态带代表 29.41%。另一方面，使用 8 个 SCoT 引物评估了 10 个埃及葫芦品种的遗传变异和关系。SCoT 分析的结果检测到 44 个扩增子，其中 50%具有多态性。此外，基于 SCoT 分析得出的相似系数构建的系统发育树的结果证实了生化分析的结果，表明最有效葫芦品种（S1 和 S2 品种）之间存在遗传关系。此外，效率较低的葫芦品种（S4 和 S5 品种）之间也存在遗传关系。这些结果可能有助于在植物育种计划中区分葫芦品种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/d6bf8a522b1c/GR2024-4182158.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/da7f7ef5391e/GR2024-4182158.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/5a5159c32bc4/GR2024-4182158.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/c99c6176b555/GR2024-4182158.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/3909161e93c8/GR2024-4182158.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/092c1319709a/GR2024-4182158.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/9f391f738d5c/GR2024-4182158.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/25b56ef6f7b6/GR2024-4182158.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/db74343ce979/GR2024-4182158.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/04b635c9ce63/GR2024-4182158.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/d6bf8a522b1c/GR2024-4182158.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/da7f7ef5391e/GR2024-4182158.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/5a5159c32bc4/GR2024-4182158.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/c99c6176b555/GR2024-4182158.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/3909161e93c8/GR2024-4182158.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/092c1319709a/GR2024-4182158.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/9f391f738d5c/GR2024-4182158.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/25b56ef6f7b6/GR2024-4182158.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/db74343ce979/GR2024-4182158.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/04b635c9ce63/GR2024-4182158.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69f2/10781529/d6bf8a522b1c/GR2024-4182158.010.jpg

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