Chauvier Adrien, Walter Nils G
Single Molecule Analysis Group and Center for RNA Biomedicine, Department of Chemistry, University of Michigan, Ann Arbor, MI, USA.
Single Molecule Analysis Group and Center for RNA Biomedicine, Department of Chemistry, University of Michigan, Ann Arbor, MI, USA.
Cell Chem Biol. 2024 Jan 18;31(1):71-85. doi: 10.1016/j.chembiol.2023.12.011. Epub 2024 Jan 10.
Commensal and pathogenic bacteria continuously evolve to survive in diverse ecological niches by efficiently coordinating gene expression levels in their ever-changing environments. Regulation through the RNA transcript itself offers a faster and more cost-effective way to adapt than protein-based mechanisms and can be leveraged for diagnostic or antimicrobial purposes. However, RNA can fold into numerous intricate, not always functional structures that both expand and obscure the plethora of roles that regulatory RNAs serve within the cell. Here, we review the current knowledge of bacterial non-coding RNAs in relation to their folding pathways and interactions. We posit that co-transcriptional folding of these transcripts ultimately dictates their downstream functions. Elucidating the spatiotemporal folding of non-coding RNAs during transcription therefore provides invaluable insights into bacterial pathogeneses and predictive disease diagnostics. Finally, we discuss the implications of co-transcriptional folding andapplications of RNAs for therapeutics and drug targets.
共生菌和致病菌不断进化，通过在不断变化的环境中有效协调基因表达水平，以在各种生态位中生存。与基于蛋白质的机制相比，通过RNA转录本本身进行调控提供了一种更快、更具成本效益的适应方式，并且可用于诊断或抗菌目的。然而，RNA可以折叠成许多复杂的、并非总是具有功能的结构，这些结构既扩展又掩盖了调控RNA在细胞内所发挥的众多作用。在这里，我们综述了关于细菌非编码RNA与其折叠途径和相互作用相关的当前知识。我们认为这些转录本的共转录折叠最终决定了它们的下游功能。因此，阐明转录过程中非编码RNA的时空折叠，可为细菌致病机制和预测性疾病诊断提供宝贵的见解。最后，我们讨论了共转录折叠的意义以及RNA在治疗和药物靶点方面的应用。
