Izar Benjamin, Mraheil Mobarak Abu, Hain Torsten
Institute of Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, Giessen 35392, Germany; E-Mails:
Int J Mol Sci. 2011;12(8):5070-9. doi: 10.3390/ijms12085070. Epub 2011 Aug 10.
Bacterial regulatory non-coding RNAs control numerous mRNA targets that direct a plethora of biological processes, such as the adaption to environmental changes, growth and virulence. Recently developed high-throughput techniques, such as genomic tiling arrays and RNA-Seq have allowed investigating prokaryotic cis- and trans-acting regulatory RNAs, including sRNAs, asRNAs, untranslated regions (UTR) and riboswitches. As a result, we obtained a more comprehensive view on the complexity and plasticity of the prokaryotic genome biology. Listeria monocytogenes was utilized as a model system for intracellular pathogenic bacteria in several studies, which revealed the presence of about 180 regulatory RNAs in the listerial genome. A regulatory role of non-coding RNAs in survival, virulence and adaptation mechanisms of L. monocytogenes was confirmed in subsequent experiments, thus, providing insight into a multifaceted modulatory function of RNA/mRNA interference. In this review, we discuss the identification of regulatory RNAs by high-throughput techniques and in their functional role in L. monocytogenes.
细菌调控性非编码RNA控制着众多的mRNA靶标，这些靶标指导着大量的生物学过程，如对环境变化的适应、生长和毒力。最近开发的高通量技术，如基因组平铺阵列和RNA测序，使得研究原核生物顺式和反式作用的调控RNA成为可能，这些RNA包括小RNA（sRNA）、反义RNA（asRNA）、非翻译区（UTR）和核糖开关。因此，我们对原核生物基因组生物学的复杂性和可塑性有了更全面的认识。在多项研究中，单核细胞增生李斯特菌被用作细胞内病原菌的模型系统，这些研究揭示了李斯特菌基因组中存在约180种调控RNA。随后的实验证实了非编码RNA在单核细胞增生李斯特菌的存活、毒力和适应机制中的调控作用，从而深入了解了RNA/mRNA干扰的多方面调节功能。在这篇综述中，我们讨论了通过高通量技术鉴定调控RNA及其在单核细胞增生李斯特菌中的功能作用。
