单分子水平下光稳定型Alexa 488的定位研究

Localization Study of Photostable Alexa 488 at Single Molecule Level.

作者信息

Bhattacharyya Tamoghna

机构信息

Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA.

Electronics and Nanoscale Engineering Division, James Watt School of Engineering, University of Glasgow, Glasgow, G12 8QQ, UK.

出版信息

J Fluoresc. 2025 Feb;35(2):955-961. doi: 10.1007/s10895-023-03580-x. Epub 2024 Jan 12.

DOI:10.1007/s10895-023-03580-x

PMID:38214847

Abstract

Understanding the relationships between molecular organization and dynamics of a complex system is very important to understand the photophysical properties of such system. This paper focuses on a novel strategy based on single molecule spectroscopy and single molecule localization microscopy to elucidate the photostability and localization of a fluorophore molecule on a 2D biomembrane. Improvement of in-plane resolution of a signal in a nano-dimension within the diffraction limit has been discussed in a new way. And, how this better in-plane resolution information can be used for precise localization of a single molecule on a 2D system has also been discussed.

摘要

理解复杂系统的分子组织与动力学之间的关系对于理解此类系统的光物理性质非常重要。本文重点介绍一种基于单分子光谱和单分子定位显微镜的新策略，以阐明二维生物膜上荧光团分子的光稳定性和定位。已以一种新的方式讨论了在衍射极限内提高纳米尺寸信号的面内分辨率。此外，还讨论了如何将这种更好的面内分辨率信息用于二维系统上单个分子的精确定位。