Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
Department of Chemistry, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Anal Chem. 2024 Jan 30;96(4):1678-1685. doi: 10.1021/acs.analchem.3c04690. Epub 2024 Jan 12.
In this paper, an electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of CA19-9 was constructed using ternary compound CdSSe nanoparticles as ECL emitter. The immunosensor employs CuS and gold-doped diindium trioxide (Au-InO) nanocubes as coreaction accelerators to achieve a double-amplification strategy. In general, a hexagonal maple leaf-shaped CuS with a large surface area was selected as the template, and the in situ growth of CdSSe on its surface was achieved using a hydrothermal method. The presence of CuS not only inhibited the aggregation of CdSSe nanoparticles to reduce their surface energy but also acted as an ECL cathode coreaction promoter, facilitating the generation of SO. Consequently, the ECL intensity of CdSSe was significantly enhanced, and the reduction potential was significantly lower. In addition, the template method was employed to synthesize Au-InO nanocubes, which offers the advantage of directly connecting materials with antibodies, resulting in a more stable construction of the immunosensor. Furthermore, InO serves as a coreaction promoter, enabling the amplification strategy for ECL intensity of CdSSe, thus contributing to the enhanced sensitivity and performance of the immunosensor. The constructed immunosensor exhibited a wide linear range (100 μU mL to 100 U mL) and a low detection limit of 80 μU mL, demonstrating its high potential and practical value for sensitive detection of CA19-9.
本文构建了一种基于三元化合物 CdSSe 纳米粒子作为电化学发光（ECL）发射器的用于超灵敏检测 CA19-9 的 ECL 免疫传感器。该免疫传感器采用 CuS 和金掺杂氧化铟（Au-InO）纳米立方体作为共反应加速剂，实现了双放大策略。通常，选择具有较大表面积的六边枫叶形 CuS 作为模板，通过水热法在其表面原位生长 CdSSe。CuS 的存在不仅抑制了 CdSSe 纳米粒子的聚集，降低了它们的表面能，而且还作为 ECL 阴极共反应促进剂，有利于 SO 的生成。因此，CdSSe 的 ECL 强度显著增强，还原电位显著降低。此外，采用模板法合成了 Au-InO 纳米立方体，其优点是可以直接将材料与抗体连接，从而使免疫传感器的构建更加稳定。此外，InO 作为共反应促进剂，为 CdSSe 的 ECL 强度放大策略提供了支持，从而提高了免疫传感器的灵敏度和性能。所构建的免疫传感器表现出较宽的线性范围（100 μU mL 至 100 U mL）和较低的检测限 80 μU mL，表明其在 CA19-9 的灵敏检测方面具有较高的潜力和实用价值。
