Ratner E N, Kamkova S P, Loseva L D, Fikhte B A
Mikrobiologiia. 1986 Sep-Oct;55(5):851-4.
The resistance to freezing-thawing was studied with Cryptococcus laurentii cultivated at a near-zero plus temperatures in a minimal or a rich medium. At the transition into the stationary phase, the resistance of the cells to freezing increased 20 times in the culture grown in the minimal medium and 8 times in the culture grown in the rich medium. Free water localization in the cell cytoplasm was determined by electron microscopy. In yeast cells with the maximal cryotolerance, free water was found mainly between glycogen granules. The authors discuss the role of glycogen as of a possible factor making the cells resistant to low temperatures.
用在接近零上温度的最低限度或丰富培养基中培养的罗伦隐球酵母研究其抗冻融性。在进入稳定期时，在最低限度培养基中生长的培养物中细胞的抗冻性增加了20倍，在丰富培养基中生长的培养物中增加了8倍。通过电子显微镜确定细胞细胞质中自由水的定位。在具有最大耐冻性的酵母细胞中，自由水主要存在于糖原颗粒之间。作者讨论了糖原作为使细胞抵抗低温的可能因素的作用。
