自噬相关蛋白 Beclin-1 和 mTOR 在吸烟和非吸烟口腔白斑病患者中的临床作用。

Clinical roles of autophagy-related proteins Beclin-1 and mTOR in smoking and non-smoking patients with oral leukoplakia.

机构信息

Department of Stomatology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai 201102, China.

Department of Stomatology, Tongling People's Hospital, Tongling 244002, Anhui Province, China.

出版信息

Afr Health Sci. 2023 Jun;23(2):616-622. doi: 10.4314/ahs.v23i2.71.

DOI:10.4314/ahs.v23i2.71

PMID:38223571

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10782313/

Abstract

BACKGROUND

To study the expressions of autophagy-related proteins Beclin-1 and mammalian target of rapamycin (mTOR) in smoking and non-smoking patients with oral leukoplakia (OLK).

METHODS

A total of 240 patients diagnosed as OLK from January 2017 to December 2017 were enrolled. Beclin-1 and mTOR expressions were detected by immunohistochemistry. Their clinical data were collected. The correlations of smoking with Beclin-1 and mTOR expressions as well as clinical factors were explored by Spearman's analysis.

RESULTS

There were significant differences in gender ratio, age, lesion location, severity and malignancy between smoking and non-smoking OLK patients (P<0.05). The positive expression rate of Beclin-1 in OLK patients with simple hyperplasia and abnormal hyperplasia in the smoking group was significantly lower than that of the non-smoking group (P<0.05). In the abnormal hyperplasia group, the number of cigarettes daily was significantly positively correlated with mTOR expression (=0.843, P=0.042). After the simple hyperplasia group was included, there was a positive correlation between smoking age and positive expression rate of mTOR (=0.942, P=0.012). For number of cigarettes and smoking age, the positive expression rates of Beclin-1 and mTOR showed significant negative correlations (=-0.952, P=0.003, =-0.953, P=0.002).

CONCLUSION

Autophagy-related proteins Beclin-1 and mTOR may be involved in the smoking-induced pathogenesis of OLK.

摘要

背景

研究自噬相关蛋白 Beclin-1 和哺乳动物雷帕霉素靶蛋白（mTOR）在吸烟和非吸烟口腔白斑（OLK）患者中的表达。

方法

选取 2017 年 1 月至 2017 年 12 月期间被诊断为 OLK 的 240 例患者，采用免疫组织化学法检测 Beclin-1 和 mTOR 的表达情况，收集其临床资料，采用 Spearman 分析探讨吸烟与 Beclin-1 和 mTOR 表达及临床因素的相关性。

结果

吸烟和非吸烟 OLK 患者的性别比、年龄、病变部位、严重程度和恶性程度差异有统计学意义（P<0.05）。单纯增生和异常增生的吸烟组 OLK 患者 Beclin-1 阳性表达率明显低于非吸烟组（P<0.05）。在异常增生组中，每日吸烟支数与 mTOR 表达呈显著正相关（=0.843，P=0.042）。纳入单纯增生组后，吸烟年龄与 mTOR 阳性表达率呈正相关（=0.942，P=0.012）。对于吸烟支数和吸烟年龄，Beclin-1 和 mTOR 的阳性表达率呈显著负相关（=-0.952，P=0.003，=-0.953，P=0.002）。