Zhang Longfei, Liu Xiaoxiao, Liu Yang, Cheng Xinyi, Xu Mingze, Qu Hengxian, Wang Wenqiong, Gu Ruixia, Chen Dawei
College of Food Science and Technology Yangzhou University Yangzhou China.
Key Laboratory of Dairy Biotechnology and Safety Control Yangzhou China.
Food Sci Nutr. 2024 Jul 28;12(10):7521-7532. doi: 10.1002/fsn3.4361. eCollection 2024 Oct.
The colonization ability of DALI02 and the promoting effect of fermented prebiotics have been studied. This study aims to evaluate the systemic immunomodulatory effects of DALI02 and DALI02 + Prebiotics on cyclophosphamide-induced immunosuppressed rats. We found that DALI02 and DALI02 + Prebiotics, especially DALI02 + Prebiotics, exhibited significant restorative effects on the immunocompromised state in rats ( < .05). Specifically, both of them promoted the recovery of body weight and immune organ function, enhanced the proliferative capacity of immune cells, and effectively reduced the levels of interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α). Furthermore, both of them significantly reduced the levels of lipopolysaccharide (LPS) and D-lactic acid in the blood ( < .05). Principal coordinate analysis (PCoA), principal component analysis (PCA), and unsupervised cluster analysis revealed that DALI02 and DALI02 + Prebiotics group were more similar to the blank group at the genus level of the gut microbiota. At the level of short-chain fatty acids (SCFAs), DALI02 + Prebiotics and blank group belonged to Cluster 3. These results suggested that the intervention with DALI02 and DALI02 + Prebiotics effectively modulated the structure of the gut microbiota, and DALI02 + Prebiotics restored the dysregulation of SCFAs. In summary, DALI02 and DALI02 + Prebiotics possess immunomodulatory functions, with the latter showing superior effects.
已对DALI02的定殖能力和发酵益生元的促进作用进行了研究。本研究旨在评估DALI02和DALI02+益生元对环磷酰胺诱导的免疫抑制大鼠的全身免疫调节作用。我们发现，DALI02和DALI02+益生元，尤其是DALI02+益生元，对大鼠的免疫受损状态表现出显著的恢复作用（<0.05）。具体而言，它们均促进了体重和免疫器官功能的恢复，增强了免疫细胞的增殖能力，并有效降低了白细胞介素6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平。此外，它们均显著降低了血液中脂多糖（LPS）和D-乳酸的水平（<0.05）。主坐标分析（PCoA）、主成分分析（PCA）和无监督聚类分析表明，在肠道微生物群的属水平上，DALI02和DALI02+益生元组与空白组更为相似。在短链脂肪酸（SCFAs）水平上，DALI02+益生元组和空白组属于聚类3。这些结果表明，用DALI02和DALI02+益生元进行干预可有效调节肠道微生物群的结构，且DALI02+益生元可恢复SCFAs的失调。总之，DALI02和DALI02+益生元具有免疫调节功能，后者效果更佳。
