Centre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, N1G 2W1, Guelph, ON, Canada.
Department of Animal Science, University of California-Davis, 95616, Davis, CA, USA.
Commun Biol. 2024 Jan 15;7(1):98. doi: 10.1038/s42003-024-05764-y.
Despite regulatory elements such as long non - coding RNAs representing most of the transcriptome, the functional understanding of long non - coding RNAs in relation to major health conditions including bovine mastitis is limited. This study examined the milk somatic cell transcriptome from udder quarters of 6 Holstein dairy cows to identify differentially expressed long non - coding RNAs using RNA - Sequencing. Ninety - four differentially expressed long non - coding RNAs are identified, 5 of which are previously annotated for gene name and length, 11 are annotated for gene name and 78 are novel, having no gene name or length previously annotated. Significant inflammatory response and regulation of immune response pathways (false discovery rate < 0.05) are associated with the differentially expressed long non - coding RNAs. QTL annotation analysis revealed 31 QTL previously annotated in the genomic regions of the 94 differentially expressed long non - coding RNAs, and the majority are associated with milk traits. This research provides a better understanding of long non - coding RNAs regulatory elements in milk somatic cells, which may enhance current breeding strategies for more adaptable or high mastitis resistant cattle.
尽管长非编码 RNA 等调控元件代表了转录组的大部分，但人们对长非编码 RNA 与包括牛乳腺炎在内的主要健康状况之间的功能关系的了解仍然有限。本研究通过 RNA 测序，检查了 6 头荷斯坦奶牛乳房各部位的牛奶体细胞转录组，以鉴定差异表达的长非编码 RNA。鉴定出 94 个差异表达的长非编码 RNA，其中 5 个具有先前注释的基因名称和长度，11 个具有注释的基因名称，78 个是新的，没有先前注释的基因名称或长度。差异表达的长非编码 RNA 与显著的炎症反应和免疫反应调节途径相关（错误发现率<0.05）。QTL 注释分析显示，在 94 个差异表达的长非编码 RNA 的基因组区域中，有 31 个先前被注释为 QTL，其中大多数与牛奶性状相关。这项研究提供了对牛奶体细胞中长非编码 RNA 调控元件的更好理解，这可能增强当前的选育策略，以获得更适应或高乳腺炎抗性的牛。
