Alqahtani Faleh, Mohamed Ali Yousif S, Almutairi Mohammed M, Alotaibi Abdullah F, Imran Imran, Alshammari Musaad A, Alshememry Abdullah K, AlSharari Shakir D, Albekairi Thamer H
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, Pakistan.
Saudi Pharm J. 2024 Jan;32(1):101895. doi: 10.1016/j.jsps.2023.101895. Epub 2023 Dec 5.
Scientific evidences reported the deleterious effect of cigarette smoking or passive smoking on brain health particularly cognitive functions, blood-brain barrier (BBB) permeability, up-regulation of inflammatory cascades, and depletion of the antioxidant system. These combined effects become more progressive in the events of stroke, traumatic brain injury (TBI), and many other neurodegenerative diseases. In the current study, we investigated the long-term administered therapeutic potential of quercetin in ameliorating the deleterious neurobiological consequences of chronic tobacco smoke exposure in TBI mice. After exposure to 21 days of cigarette smoke and treatment with 50 mg/kg of quercetin, C57BL/6 mice were challenged for the induction of TBI by the weight drop method. Subsequently, a battery of behavioral tests and immunohistochemical analyses revealed the beneficial effect of quercetin on the locomotive and cognitive function of TBI + smoked group mice (p < 0.05 vs control sham). Immunohistochemistry analysis (Nrf2, HO-1, NFkB, caspase 3) demonstrated a marked protection after 21 days of quercetin treatment in the chronic tobacco smoking group possibly by up-regulation of antioxidant pathways, and decreased apoptosis. In conclusion, our findings support the therapeutic effectiveness of quercetin in partly protecting the central neurological functions that become aberrantly impaired in combined habitual cigarette-smoking individuals impacted with TBI.
科学证据表明，吸烟或被动吸烟对大脑健康具有有害影响，尤其是对认知功能、血脑屏障（BBB）通透性、炎症级联反应上调以及抗氧化系统耗竭方面。在中风、创伤性脑损伤（TBI）和许多其他神经退行性疾病中，这些综合影响会变得更加严重。在本研究中，我们调查了槲皮素长期给药对改善TBI小鼠慢性烟草烟雾暴露有害神经生物学后果的治疗潜力。在暴露于香烟烟雾21天并给予50mg/kg槲皮素治疗后，通过重物坠落法对C57BL/6小鼠进行TBI诱导挑战。随后，一系列行为测试和免疫组织化学分析显示，槲皮素对TBI+吸烟组小鼠的运动和认知功能具有有益作用（与对照假手术组相比，p<0.05）。免疫组织化学分析（Nrf2、HO-1、NFkB、caspase 3）表明，在慢性吸烟组中，槲皮素治疗21天后具有显著保护作用，可能是通过上调抗氧化途径和减少细胞凋亡实现的。总之，我们的研究结果支持槲皮素在部分保护受TBI影响的习惯性吸烟个体中异常受损的中枢神经功能方面的治疗有效性。
