关于溴化乙锭与DNA的协同结合和非协同结合
On the cooperative and noncooperative binding of ethidium to DNA.
作者信息
Winkle S A, Rosenberg L S, Krugh T R
出版信息
Nucleic Acids Res. 1982 Dec 20;10(24):8211-23. doi: 10.1093/nar/10.24.8211.
Abstract
The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other hand, no evidence of cooperative binding was observed in the binding isotherms obtained with calf thymus, C. perfringens, M. lysodeikticus, or poly(dG-dC) X (dG-dC) under the experimental conditions used (0.1 M NaCl).
摘要
通过相分配和直接分光光度技术研究了溴化乙锭与天然DNA以及聚（dG-dC）×聚（dG-dC）的平衡结合。从这两种实验技术获得的结合等温线表明，溴化乙锭以协同方式与大肠杆菌DNA结合。另一方面，在所使用的实验条件（0.1 M NaCl）下，用小牛胸腺、产气荚膜梭菌、溶壁微球菌或聚（dG-dC）×聚（dG-dC）获得的结合等温线中未观察到协同结合的证据。
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