Department of Genetics and Plant Production, Aula Dei Experimental Station (EEAD-CSIC), Avda. Montañana 1005, E-50059 Zaragoza, Spain.
Syngenta Seeds SAS, 12 Chemin de l'Hobit, 31790 Saint Sauveur, France.
Sensors (Basel). 2020 Mar 8;20(5):1486. doi: 10.3390/s20051486.
Crop productivity can be expressed as the product of the amount of radiation intercepted, radiation use efficiency and harvest index. Genetic variation for components of radiation use efficiency has rarely been explored due to the lack of appropriate equipment to determine parameters at the scale needed in plant breeding. On the other hand, responses of the photosynthetic apparatus to environmental conditions have not been extensively investigated under field conditions, due to the challenges posed by the fluctuating environmental conditions. This study applies a rapid, low-cost, and reliable high-throughput phenotyping tool to explore genotypic variation for photosynthetic performance of a set of hybrid barleys and their parents under mild water-stress and unstressed field conditions. We found differences among the genotypic sets that are relevant for plant breeders and geneticists. Hybrids showed lower leaf temperature differential and higher non-photochemical quenching, resembling closer the male parents. The combination of traits detected in hybrids seems favorable, and could indicate improved photoprotection and better fitness under stress conditions. Additionally, we proved the potential of a low-cost, field-based phenotyping equipment to be used routinely in barley breeding programs for early screening for stress tolerance.
作物生产力可以表示为截获辐射量、辐射利用效率和收获指数的乘积。由于缺乏在植物育种所需规模上确定参数的适当设备，因此很少探索辐射利用效率组成部分的遗传变异。另一方面，由于环境条件的波动带来的挑战，光合作用器官对环境条件的响应在田间条件下尚未得到广泛研究。本研究应用一种快速、低成本且可靠的高通量表型分析工具，来探索一组杂交大麦及其亲本在轻度水分胁迫和无胁迫田间条件下的光合性能的基因型变异。我们发现了与植物育种家和遗传学家相关的基因型差异。杂种的叶片温差较低，非光化学猝灭较高，与雄性亲本更相似。在杂种中检测到的性状组合似乎有利，并且可能表明在胁迫条件下具有更好的光保护和适应性。此外，我们证明了低成本、基于田间的表型分析设备的潜力，可以在大麦育种计划中常规用于早期筛选耐胁迫性。
