土耳其小菜蛾种群的遗传多样性。（鳞翅目：菜蛾科）
Genetic diversity of diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) populations in Türkiye.
机构信息
Faculty of Agriculture, Department of Agricultural Biotechnology, Çanakkale Onsekiz Mart University, Çanakkale, 17100, Turkey.
出版信息
Mol Biol Rep. 2024 Jan 18;51(1):146. doi: 10.1007/s11033-023-08928-x.
BACKGROUND
Diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important worldwide pest of plants belonging to the Brassicaceae family. In this study, we investigated genetic diversity of DBM populations in Brassicaceae production areas in Türkiye using the partial mtDNA CO1 gene region.
METHODS
We determined 43 samples from 11 different populations for haplotype variations using the partial mitochondrial DNA sequences a 684 bp fragment of the CO1 gene.
RESULTS
The results indicated that, the average haplotype diversity (Hd) was determined as 0.962 and nucleotide diversity (π) was determined as 0.557%. In neutrality tests, negative values were obtained in Tajima's D and Fu' Fs tests (Fu' Fs=-0.40, Tajima's D=-0.01). Tajima's D test was not found significant (p > 0.05). Fst value among DBM population estimates ranged from 0 to 0.631. Barcode gap distance was determined as 1.6%, but the intraspecies of genetic distance were found to be 0.15%.
CONCLUSION
In conclusion, the presented study provided detailed and fundamental information about the genetic diversity of DBM populations in Türkiye. Further studies are needed to develop alternative pest management strategies for DBM populations integrating genetic approaches.
背景
小菜蛾（DBM），也称粉纹夜蛾，隶属于鳞翅目菜蛾科，是一种全球性的十字花科植物重要害虫。本研究利用部分 mtDNA CO1 基因区域，调查了土耳其十字花科作物产区小菜蛾种群的遗传多样性。
方法
我们通过 684bp 的 CO1 基因片段，对来自 11 个不同种群的 43 个样本进行了部分线粒体 DNA 序列的单倍型变异分析。
结果
结果表明，平均单倍型多样性（Hd）为 0.962，核苷酸多样性（π）为 0.557%。在中性检验中，Tajima 的 D 和 Fu' Fs 检验均得到负值（Fu' Fs=-0.40，Tajima 的 D=-0.01）。Tajima 的 D 检验不显著（p>0.05）。小菜蛾种群间的 Fst 值范围为 0 至 0.631。条码间隙距离确定为 1.6%，但种内遗传距离为 0.15%。
结论
综上所述，本研究为土耳其小菜蛾种群的遗传多样性提供了详细和基本的信息。需要进一步研究，以整合遗传方法，为小菜蛾种群制定替代害虫管理策略。
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