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从一只奶山羊的乳腺中检测到贝氏柯克斯体。

Detection of Coxiella burnetii in the mammary gland of a dairy goat.

机构信息

University of Veterinary Medicine Hannover, Foundation, Clinic for Swine and Small Ruminants, Bischofsholer Damm 15, Hannover, 30173, Germany.

Chemisches und Veterinäruntersuchungsamt Westfalen, Zur Taubeneiche 10-12, Arnsberg, 59821, Germany.

出版信息

Vet Res Commun. 2024 Jun;48(3):1341-1352. doi: 10.1007/s11259-023-10233-8. Epub 2024 Jan 18.

DOI:10.1007/s11259-023-10233-8
PMID:38236458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11147866/
Abstract

The zoonotic bacterium Coxiella (C.) burnetii can be excreted by infected goats through birth products and milk. The detection of C. burnetii DNA in the mammary gland tissue of infected dairy goats and intermittent milk shedders has been reported, but confirmation of C. burnetii bacteria in the udder remained pending. The pathogen caused abortions in a 152-head dairy goat herd, resulting in the vaccination against C. burnetii of the entire herd with annual boosters. To monitor the C. burnetii shedding at herd level, monthly bulk tank milk (BTM) samples were analyzed using PCR (IS1111). Despite vaccination, C. burnetii DNA was detected in BTM samples within the first 16 months of the study. Therefore, individual milk samples were tested on four different occasions several months apart to identify potential intermittent milk shedders. Only one goat (#67455) tested positive three times. This goat was necropsied to investigate the presence of C. burnetii in the udder and other organs. PCR detected C. burnetii DNA solely in both mammary glands and the left teat cistern. Immunohistological examination identified C. burnetii antigen in mammary gland tissue, confirmed by the detection of C. burnetii bacteria in the mammary epithelial cells using fluorescence in situ hybridization. The removal of goat #67455 led to negative BTM samples until the end of the study. The findings demonstrate the occurrence of C. burnetii in the mammary gland of a naturally infected and vaccinated goat. The presence possibly contributed to intermittent milk shedding of goat #67455, and the mammary gland tissue may serve as a replicative niche for C. burnetii.

摘要

动物源性细菌贝氏柯克斯体（Coxiella）（C.）可通过感染山羊的产道和乳汁排出。已报道在感染的奶山羊乳腺组织和间歇性乳汁排放者中检测到 C. burnetii DNA，但尚未确认乳房中有 C. burnetii 细菌。该病原体导致 152 头奶山羊群流产，因此对整个羊群进行了 C. burnetii 疫苗接种，并每年加强免疫。为了监测畜群水平的 C. burnetii 脱落情况，每月使用 PCR（IS1111）分析大容量奶罐奶（BTM）样品。尽管进行了疫苗接种，但在研究的前 16 个月内仍在 BTM 样品中检测到 C. burnetii DNA。因此，在相隔数月的四个不同时间点对个体牛奶样品进行了测试，以确定潜在的间歇性乳汁排放者。只有一只山羊（#67455）三次检测呈阳性。对这只山羊进行了剖检，以调查 C. burnetii 在乳房和其他器官中的存在情况。PCR 仅在两个乳腺和左侧乳池检测到 C. burnetii DNA。免疫组织化学检查在乳腺组织中发现了 C. burnetii 抗原，通过荧光原位杂交检测到乳腺上皮细胞中的 C. burnetii 细菌进一步证实了这一点。移除山羊#67455 后，直到研究结束，BTM 样本均为阴性。这些发现表明，在自然感染和接种疫苗的山羊的乳腺中发生了 C. burnetii。这种存在可能导致了山羊#67455 的间歇性乳汁排放，乳腺组织可能是 C. burnetii 的复制生态位。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/0d4a0108efd2/11259_2023_10233_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/58080333e4dd/11259_2023_10233_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/76e3a123efeb/11259_2023_10233_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/91d013411426/11259_2023_10233_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/4548922682b7/11259_2023_10233_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/1f3d00a4d506/11259_2023_10233_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/0d4a0108efd2/11259_2023_10233_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/58080333e4dd/11259_2023_10233_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/76e3a123efeb/11259_2023_10233_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/91d013411426/11259_2023_10233_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/4548922682b7/11259_2023_10233_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/1f3d00a4d506/11259_2023_10233_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3f9/11147866/0d4a0108efd2/11259_2023_10233_Fig6_HTML.jpg

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