Liang Jieying, Chen Qianfan, Yong Joel, Suyama Hiroki, Biazik Joanna, Njegic Bosiljka, Rawal Aditya, Liang Kang
School of Chemical Engineering and Australian Centre for NanoMedicine, The University of New South Wales Sydney NSW 2052 Australia
Graduate School of Biomedical Engineering, The University of New South Wales Sydney NSW 2052 Australia.
Chem Sci. 2023 Dec 13;15(3):991-1002. doi: 10.1039/d3sc04973f. eCollection 2024 Jan 17.
We present a novel study introducing a durable and robust covalent-organic framework (COF) nanocoating, developed on living cells. This COF nanocoating demonstrates remarkable resistance against a diverse range of lethal stressors, including high temperature, extreme pH, ultraviolet radiation, toxic metal ions, organic pollutants, and strong oxidative stress. Notably, the nanocoating exhibits exceptional cell survival enhancement under high temperature and strongly acidic conditions, an aspect yet unexplored in the case of metal-organic framework nanocoatings and other nanomaterials. Moreover, functionalization of the nanocoating with an exogenous enzyme catalase enables yeast fermentation and ethanol production even under strong oxidative stress. Our findings establish the durable and robust COF nanocoating as a reliable platform for safeguarding vulnerable microorganisms to allow their utilisation in a wide range of adverse environments.
我们展示了一项新颖的研究，介绍了一种在活细胞上开发的耐用且坚固的共价有机框架（COF）纳米涂层。这种COF纳米涂层对多种致命应激源具有显著抗性，包括高温、极端pH值、紫外线辐射、有毒金属离子、有机污染物和强氧化应激。值得注意的是，该纳米涂层在高温和强酸性条件下表现出卓越的细胞存活增强能力，这在金属有机框架纳米涂层和其他纳米材料的情况下尚未被探索。此外，用外源酶过氧化氢酶对纳米涂层进行功能化，即使在强氧化应激下也能实现酵母发酵和乙醇生产。我们的研究结果确立了这种耐用且坚固的COF纳米涂层作为一个可靠的平台，用于保护脆弱的微生物，使其能够在广泛的恶劣环境中得到利用。
