de Lima Lucas F, Ferreira André L, Ranjan Ishani, Collman Ronald G, de Araujo William R, de la Fuente-Nunez Cesar
Machine Biology Group, Departments of Psychiatry and Microbiology, Institute for Biomedical Informatics, Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Departments of Bioengineering and Chemical and Biomolecular Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA.
Cell Rep Phys Sci. 2023 Aug 16;4(8). doi: 10.1016/j.xcrp.2023.101476.
COVID-19 has led to over 6.8 million deaths worldwide and continues to affect millions of people, primarily in low-income countries and communities with low vaccination coverage. Low-cost and rapid response technologies that enable accurate, frequent testing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are crucial for outbreak prevention and infectious disease control. Here we produce and characterize cellulose fibers naturally generated by the bacterium as an alternative biodegradable substrate for manufacturing an eco-friendly diagnostic test for COVID-19. Using this green technology, we describe a novel and label-free potentiometric diagnostic test that can detect SARS-CoV-2 within 10 min and costs US$3.50 per unit. The test has bacterial cellulose (BC) as its substrate and a carbon-based electrode modified with graphene oxide and the human angiotensin-converting enzyme-2 (ACE2) as its receptor. Our device accurately and precisely detects emerging SARS-CoV-2 variants and demonstrates exceptional sensitivity, specificity, and accuracy for tested clinical nasopharyngeal/oropharyngeal (NP/OP) samples.
新冠病毒已导致全球超过680万人死亡，并且仍在影响数百万人，主要是在低收入国家以及疫苗接种覆盖率低的社区。能够对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）变体进行准确、频繁检测的低成本和快速响应技术对于疫情预防和传染病控制至关重要。在此，我们制备并表征了由细菌天然产生的纤维素纤维，作为制造一种用于新冠病毒的环保诊断测试的替代可生物降解底物。使用这种绿色技术，我们描述了一种新型的无标记电位诊断测试，该测试可在10分钟内检测出SARS-CoV-2，每单位成本为3.50美元。该测试以细菌纤维素（BC）为底物，以用氧化石墨烯和人血管紧张素转换酶2（ACE2）修饰的碳基电极作为其受体。我们的设备能够准确、精确地检测出新兴的SARS-CoV-2变体，并且对测试的临床鼻咽/口咽（NP/OP）样本表现出卓越的灵敏度、特异性和准确性。
