de Lima Lucas F, Ferreira André L, Dos Santos Letícia Ester, Coelho Keyla Lívian P, Santos Keyla Teixeira, Schmidt Ariane, de Jesus Marcelo Bispo, Paixão Thiago R L C, de Araujo William R
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
Laboratório de Sensores Químicos Portáteis, Departamento de Química Analítica, Instituto de Química, Universidade Estadual de Campinas - UNICAMP, Campinas, SP 13083-861, Brazil.
ACS Appl Bio Mater. 2025 Jul 21;8(7):6339-6349. doi: 10.1021/acsabm.5c00825. Epub 2025 Jun 29.
The development of sustainable and high-performance electrochemical sensors is crucial for advancing biomedical applications. In this work, we introduce a hydrogen peroxide (HO) sensor based on bacterial cellulose-derived laser-scribed graphene (BC-LSG), modified with MXene and platinum nanoparticles (PtNPs). Bacterial cellulose (BC), a biodegradable and renewable material, was cultivated and transformed into a highly conductive carbon network using CO laser irradiation, producing a flexible, portable, and miniaturized electrochemical platform. The incorporation of MXene and PtNPs significantly enhanced the electrocatalytic response toward HO oxidation, achieving a wide linear concentration range (15-95 μmol L) and a low detection limit (0.35 μmol L). Compared to traditional enzymatic sensors, our nanostructured BC-LSG device offers superior stability, reproducibility, and eco-friendliness, aligning with green analytical chemistry principles. The sensor was successfully applied for HO detection in mammalian cells, demonstrating its potential for real-time monitoring of oxidative stress, a key biomarker in cancer progression and therapeutic responses. This work underscores the synergy between biopolymeric materials, nanotechnology, and laser processing, opening new avenues for scalable, disposable, and sustainable electrochemical devices.
开发可持续且高性能的电化学传感器对于推动生物医学应用至关重要。在这项工作中，我们介绍了一种基于细菌纤维素衍生的激光刻写石墨烯（BC-LSG）的过氧化氢（HO）传感器，该传感器用MXene和铂纳米颗粒（PtNPs）进行了修饰。细菌纤维素（BC）是一种可生物降解的可再生材料，通过CO激光辐照培养并转化为高导电碳网络，从而产生了一个灵活、便携且小型化的电化学平台。MXene和PtNPs的加入显著增强了对HO氧化的电催化响应，实现了宽线性浓度范围（15 - 95 μmol L）和低检测限（0.35 μmol L）。与传统酶传感器相比，我们的纳米结构BC-LSG装置具有卓越的稳定性、重现性和生态友好性，符合绿色分析化学原理。该传感器已成功应用于哺乳动物细胞中HO的检测，证明了其对氧化应激进行实时监测的潜力，氧化应激是癌症进展和治疗反应中的关键生物标志物。这项工作强调了生物聚合物材料、纳米技术和激光加工之间的协同作用，为可扩展、一次性和可持续的电化学装置开辟了新途径。
