Gilbert T, Lelievre-Pegorier M, Malienou R, Meulemans A, Merlet-Benichou C
Toxicology. 1987 Mar;43(3):301-13. doi: 10.1016/0300-483x(87)90089-8.
Pregnant rats were injected daily, from the 10th day of gestation to term, with 75 mg/kg of gentamicin. They gave birth about 15 h later than control pregnant rats injected with saline to pups with various degrees of growth retardation. In pups born of gentamicin-treated mothers, the number of nephrons present at birth, as well as the final number of nephrons, were reduced by at least 20%. Observation of the kidneys by light microscopy showed focal tubular lesions on the mature nephrons. The intrarenal concentration of gentamicin was higher in the severely growth retarded pups than in the others. In another series of experiments, rats were given 75 mg/kg of gentamicin daily from days 1 to 13 after birth. Although under these conditions the concentration of gentamicin reached in the postnatal kidney was higher than that reached after exposure in utero, no reduction of the final number of nephrons was observed. It is concluded that administration of gentamicin to pregnant rats caused focal tubular lesions in the developing kidney and a reduced rate of early nephrogenesis. The latter was probably due to growth retardation, though a more direct effect of gentamicin on early nephrogenesis may also have been involved.
从妊娠第10天至足月，每天给怀孕大鼠注射75毫克/千克庆大霉素。它们比注射生理盐水的对照怀孕大鼠晚约15小时产仔，所产幼崽有不同程度的生长迟缓。在接受庆大霉素治疗的母鼠所生的幼崽中，出生时存在的肾单位数量以及最终的肾单位数量至少减少了20%。光镜观察肾脏显示成熟肾单位有局灶性肾小管病变。严重生长迟缓的幼崽肾内庆大霉素浓度高于其他幼崽。在另一系列实验中，从出生后第1天至第13天，每天给大鼠注射75毫克/千克庆大霉素。尽管在这些条件下，出生后肾脏中达到的庆大霉素浓度高于子宫内接触后的浓度，但未观察到肾单位最终数量减少。得出的结论是，给怀孕大鼠注射庆大霉素会导致发育中的肾脏出现局灶性肾小管病变，并降低早期肾发生的速率。后者可能是由于生长迟缓，不过庆大霉素对早期肾发生的更直接作用可能也有参与。
