Mechanistic analysis of Th2-type inflammatory factors in asthma.

BACKGROUND

The main pathological features of asthma are widespread chronic inflammation of the airways and restricted ventilation due to airway remodeling, which involves changes in a range of regulatory pathways. While the role of T helper type 2 (Th2)-related inflammatory factors in this process is known, the detailed understanding of how genes affect protein functions during airway remodeling is still lacking. This study aims to fill this knowledge gap by integrating gene expression data and protein function analysis, providing new scientific insights for a deeper understanding of the mechanisms of airway remodeling and for further development of asthma treatment strategies.

METHODS

In this study, the mechanism of Th2-related inflammatory factors in tracheal remodeling was studied through differentially expressed gene (DEG) screening, enrichment analysis, protein-protein interaction (PPI) network construction, machine learning, and the construction of a line graph model.

RESULTS

Our study revealed that , , , , , , , , and may be the key genes for airway remodeling; epithelial-mesenchymal transition (EMT)-related genes , , , and may be involved in airway remodeling due to asthma; may affect the occurrence of airway remodeling by binding to UBE2C protein or by regulating genes, respectively; and may affect the occurrence of airway remodeling by regulating the downstream Toll-like receptor (TLR) signaling pathway and thus and may influence the occurrence of tracheal remodeling by regulating downstream TLR signaling pathways.

CONCLUSIONS

This study further mined the asthma gene microarray database through bioinformatics analysis and identified key genes and important pathways affecting airway remodeling in asthma patients, providing new ideas to uncover the mechanism of airway remodeling due to asthma and then seek new therapeutic targets.