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一系列用于人类来源鉴定和ABO血型分组的微量人类血迹检测。

A sequence of tests of minute human blood stains for human origin identification and ABO blood grouping.

作者信息

Tokiwa K

出版信息

Z Rechtsmed. 1986;97(3):157-64. doi: 10.1007/BF00201238.

DOI:10.1007/BF00201238
PMID:3825313
Abstract

A series of examinations is presented for human origin identification and ABO blood grouping of doubtful minute human blood stains. A blood-stained thread (0.5 cm in length) was first tested to identify human origin by microprecipitation method and then the ABO blood type was determined by both a modified absorption-elution test and a modified mixed agglutination. In the continuous tests, the maximum limits of positive reactions of the microprecipitation method, the modified absorption-elution test, and the modified mixed agglutination were 1:640, 1:160, and 1:2,560 diluted blood, respectively. A and B agglutinogens were more sensitively determined than H agglutinogen. Hemagglutinogens of blood stains on cotton threads were more easily detected than those of polyester ones.

摘要

本文介绍了一系列用于对可疑微小人类血迹进行人类来源鉴定和ABO血型分型的检测方法。首先,采用微量沉淀法对一段沾血的线(长度为0.5厘米)进行人类来源鉴定,然后通过改良的吸收-洗脱试验和改良的混合凝集试验来确定ABO血型。在连续测试中,微量沉淀法、改良的吸收-洗脱试验和改良的混合凝集试验的阳性反应最大稀释倍数分别为1:640、1:160和1:2560。A和B凝集原比H凝集原更容易被检测到。棉线上血迹的血凝原比聚酯线上的更容易被检测到。

相似文献

1
A sequence of tests of minute human blood stains for human origin identification and ABO blood grouping.一系列用于人类来源鉴定和ABO血型分组的微量人类血迹检测。
Z Rechtsmed. 1986;97(3):157-64. doi: 10.1007/BF00201238.
2
[Specimen preparation for ABO determination of various kinds of blood stains].
Arch Kriminol. 1989 Jul-Aug;184(1-2):38-47.
3
[Micromethod for the detection of erythrocyte antigens in blood stains].
Z Rechtsmed. 1986;96(3):163-71. doi: 10.1007/BF00200276.
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[Determination of subgroups A1-A2 in blood stains].[血痕中A1 - A2亚群的测定]
Soud Lek. 1980;25 Suppl 3:44-5.
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ABO blood grouping of bloodstains by sandwich ELISA using monoclonal antibody specific for human red cell band 3.
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A microplate method for reverse ABO typing of bloodstains.一种用于血迹ABO血型反向定型的微孔板法。
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[Study of antigens of the ABO system in blood stains with monoclonal antisera].
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Errors in ABO typing of blood stains using PCR.
Int J Legal Med. 2000;113(6):352-5. doi: 10.1007/s004149900127.
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[Use of proteases to improve the sensitivity of ABO antigen detection by absorption-elution test in analysis of human excretion traces].
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10
[Sensitive identification of human blood and simultaneous determination of ABO blood group from a minute bloodstain by an ELISA-ABC method].[应用酶联免疫吸附测定-抗生物素蛋白-生物素复合物法从微量血迹中灵敏鉴定人血并同时测定ABO血型]
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引用本文的文献

1
Comparative studies between counterimmunoelectrophoresis and microprecipitation method of identification of human minute bloodstains.对流免疫电泳法与微量沉淀法鉴定人体微量血迹的比较研究
Z Rechtsmed. 1988;99(4):227-33. doi: 10.1007/BF00204432.
2
Rapid and sensitive identification of human blood by an ELISA-ABC method using a biotinylated antibody against human HbA0.使用抗人血红蛋白A0生物素化抗体的ELISA-ABC法对人血进行快速灵敏鉴定。
Z Rechtsmed. 1990;103(5):329-34. doi: 10.1007/BF01263037.

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