Taki K
Tohoku J Exp Med. 1976 May;119(1):71-7. doi: 10.1620/tjem.119.71.
HbA1 weight in individual red cells, amounting to 13,746, was quantitatively determined in 72 male and 17 female healthy adults. The red cells were besmeared on the anti-HbA1 serum agarose plate and the precipitation rings formed were measured from the size printed on sheets of printing paper. HbA1 weight in individual cells was calculated from the calibration curve prepared specially for HbA1, weight in a red cell and the volume of a precipitation ring. HbA1 weight in individual cells ranged from 4.7 to 62.6 pg and averaged 23.7 pg. The mean HbA1 weight in individual cells per head ranged from 18.1 to 34.0 pg and averaged 26.4+/-4.3 pg. No difference in the mean HbA1 weight was found between male and female adults, and among the age groups of them. Average ratio of the mean HbA1 weight in individual cells to the total hemoglobin in the average red cell was 87.4%. The HbA1 weight in the average red cell was determined from HbA1 concentration and red cell counts of the whole blood. The correlation coefficient between the mean HbA1 weight in individual cells and the HbA1 weight in the average red cell was 0.546 (p less than 0.001).
对72名男性和17名女性健康成年人的单个红细胞中的糖化血红蛋白A1（HbA1）重量（总计13746个）进行了定量测定。将红细胞涂抹在抗HbA1血清琼脂糖平板上，并根据打印纸上打印的尺寸测量形成的沉淀环。单个细胞中的HbA1重量根据专门为HbA1制备的校准曲线、单个红细胞中的重量以及沉淀环的体积来计算。单个细胞中的HbA1重量范围为4.7至62.6皮克，平均为23.7皮克。每人单个细胞中的平均HbA1重量范围为18.1至34.0皮克，平均为26.4±4.3皮克。在成年男性和女性之间以及他们的年龄组之间，未发现平均HbA1重量有差异。单个细胞中平均HbA1重量与平均红细胞中总血红蛋白的平均比率为87.4%。平均红细胞中的HbA1重量根据全血的HbA1浓度和红细胞计数来确定。单个细胞中平均HbA1重量与平均红细胞中HbA1重量之间的相关系数为0.546（p＜0.001）。
