Department of Integrated Traditional Chinese and Western Medicine, Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, 410008, P.R. China.
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, P.R. China.
BMC Complement Med Ther. 2024 Jan 22;24(1):48. doi: 10.1186/s12906-023-04314-z.
Osteoarthritis (OA) is a severe chronic inflammatory disease. As the main active component of Astragalus mongholicus Bunge, a classic traditional ethnic herb, calycosin exhibits anti-inflammatory action and its mechanism of exact targets for OA have yet to be determined. In this study, we established an anterior cruciate ligament transection (ACLT) mouse model. Mice were randomized to sham, OA, and calycosin groups. Cartilage synthesis markers type II collagen (Col-2) and SRY-Box Transcription Factor 9 (Sox-9) increased significantly after calycosin gavage. While cartilage matrix degradation index cyclooxygenase-2 (COX-2), phosphor-epidermal growth factor receptor (p-EGFR), and matrix metalloproteinase-9 (MMP9) expression were decreased. With the help of network pharmacology and molecular docking, these results were confirmed in chondrocyte ADTC5 cells. Our results indicated that the calycosin treatment significantly improved cartilage damage, this was probably attributed to reversing the imbalance between chondrocyte synthesis and catabolism.
骨关节炎（OA）是一种严重的慢性炎症性疾病。作为蒙古黄芪（Astragalus mongholicus Bunge）的主要活性成分，一种经典的传统民族草药，毛蕊异黄酮具有抗炎作用，但其确切的 OA 靶点机制尚待确定。在这项研究中，我们建立了前交叉韧带切断（ACLT）小鼠模型。将小鼠随机分为假手术组、OA 组和毛蕊异黄酮组。毛蕊异黄酮灌胃后，软骨合成标志物 II 型胶原（Col-2）和性别决定区 Y 框转录因子 9（Sox-9）明显增加。而软骨基质降解指标环氧化酶-2（COX-2）、磷酸化表皮生长因子受体（p-EGFR）和基质金属蛋白酶-9（MMP9）的表达则降低。借助网络药理学和分子对接，在软骨细胞 ADTC5 细胞中证实了这些结果。我们的研究结果表明，毛蕊异黄酮治疗可显著改善软骨损伤，这可能归因于逆转软骨细胞合成和分解代谢之间的失衡。
