Muñoz-Molina Nicolás, Parada Javier, Simirgiotis Mario, Montecinos-González Romina
Graduate School, Faculty of Agricultural and Food Sciences, Universidad Austral de Chile, Valdivia 5090000, Chile.
Institute of Food Science and Technology, Faculty of Agricultural and Food Sciences, Universidad Austral de Chile, Valdivia 5090000, Chile.
Foods. 2024 Jan 15;13(2):269. doi: 10.3390/foods13020269.
The world's population is in a demographical transition, with an increase in the number of older adults and prevalence of diseases related to aging. This study evaluated in vitro the potential of using extract (extracted using ultrasound-assisted extraction) to inhibit key enzymes associated with the development of age-related diseases. Our results show that an extract extracted via ultrasound-assisted extracted, as well as an extract conventional extracted from presented antidiabetes potential by exhibiting inhibitory activity against α-glucosidase (91.8 ± 1.0% and 93.8 ± 0.3%, respectively, at 500 µg/mL) and α-amylase (42.2 ± 1.4% and 61.9 ± 0.9%, respectively, at 1500 µg/mL) enzymes related to starch digestion and postprandial glycemic response. Also, the extracts showed inhibitory activity against the enzymes acetylcholinesterase (51.5% and 50.8%, respectively, at 500 µg/mL) and butyrylcholinesterase (32.8% and 34.4%, respectively, at 0.5 mg/mL), the biomarkers associated with Alzheimer's disease, and angiotensin-converting enzyme (98.7 ± 7.4% and 93.0 ± 3.4%, respectively, at 2.0 mg/mL), which is key in the regulation of vascular tone and blood pressure and helps to prevent the development of hypertension. In conclusion, the extract of obtained from ultrasound-assisted extraction has the potential to prevent the development of age-related pathologies such as diabetes, Alzheimer's disease, and hypertension.
世界人口正处于人口结构转型期，老年人数量增加，与衰老相关的疾病患病率上升。本研究在体外评估了使用[提取物名称]提取物（采用超声辅助提取法提取）抑制与年龄相关疾病发展相关关键酶的潜力。我们的结果表明，通过超声辅助提取得到的提取物以及从[原料名称]传统提取得到的提取物，通过对α-葡萄糖苷酶（在500μg/mL时分别为91.8±1.0%和93.8±0.3%）和α-淀粉酶（在1500μg/mL时分别为42.2±1.4%和61.9±0.9%）表现出抑制活性，呈现出抗糖尿病潜力，这两种酶与淀粉消化和餐后血糖反应有关。此外，提取物对乙酰胆碱酯酶（在500μg/mL时分别为51.5%和50.8%）和丁酰胆碱酯酶（在0.5mg/mL时分别为32.8%和34.4%）这两种与阿尔茨海默病相关的生物标志物以及血管紧张素转换酶（在2.0mg/mL时分别为98.7±7.4%和93.0±3.4%）表现出抑制活性，血管紧张素转换酶在血管张力和血压调节中起关键作用，有助于预防高血压的发展。总之，通过超声辅助提取获得的[提取物名称]提取物有潜力预防糖尿病、阿尔茨海默病和高血压等与年龄相关疾病的发展。
