Yu Yang, Andreev Andrey Yurevich, Rogovaya Olga Sergeevna, Subbot Anastasia Mikhailovna, Domogatsky Sergey Petrovich, Avetisov Sergey Eduardovich, Vorotelyak Ekaterina Andreevna, Osidak Egor Olegovich
Department of Eye Diseases, I.M. Sechenov First Moscow State Medical University, 8-2, Trubetskaya Street, 119991 Moscow, Russia.
Department of Pathologies of Optical Medium of the Eye, Krasnov Research Institute of Eye Diseases, 11A Rossolimo St., 119021 Moscow, Russia.
Biomedicines. 2024 Jan 3;12(1):101. doi: 10.3390/biomedicines12010101.
With the development of regenerative medicine in ophthalmology, the identification of cells with high proliferative potential in the limbal area has attracted the attention of ophthalmologists and offered a new option for treatment in clinical practice. Limbal stem cell deficiency (LSCD) is an identified eye disease with a difficult and negative outcome, for which the traditional treatment is keratoplasty. This study sought to evaluate the efficacy of matrix-assisted cell transplantation consisting of in vitro-cultured autologous limbal stem cells (LSCs) and type I collagen for the treatment of LSCD in rabbits. LSCD was induced in 10 rabbits by a combination of mechanical limbectomy and alkali burns. Cells were cultured on a plate for 14 days before being transferred to a collagen-based matrix for another 7 days. Rabbits were divided into two groups as follows: the experimental group (five rabbits) received matrix-assisted cell transplantation, while the control group (five rabbits) received only conservative therapy with anti-inflammatory eye drops. During the postoperative period, all rabbits were examined using slit-lamp biomicroscopy with photo-registration and fluorescent staining, impression cytology and anterior segment optical coherence tomography (AS-OCT). Rabbits were euthanized at 30 and 120 days, and their corneas were processed for histology and immunohistochemistry. As a consequence, rabbits in the experimental group demonstrated the restoration of the corneal epithelium and transparency without epithelial defects. Moreover, goblet cells were absent in the central zone of the corneal epithelium. In conclusion, our new method of treatment enhanced the corneal surface and is an effective method of treatment for LSCD in rabbits.
随着眼科再生医学的发展，角膜缘区域具有高增殖潜力细胞的鉴定引起了眼科医生的关注，并为临床治疗提供了新的选择。角膜缘干细胞缺乏症（LSCD）是一种已明确的眼病，治疗困难且预后不良，传统治疗方法是角膜移植术。本研究旨在评估由体外培养的自体角膜缘干细胞（LSCs）和I型胶原蛋白组成的基质辅助细胞移植治疗兔LSCD的疗效。通过机械去肢术和碱烧伤联合诱导10只兔发生LSCD。细胞在平板上培养14天，然后转移到基于胶原蛋白的基质中再培养7天。将兔分为两组如下：实验组（5只兔）接受基质辅助细胞移植，而对照组（5只兔）仅接受抗炎眼药水的保守治疗。术后期间，所有兔均使用带照片记录的裂隙灯生物显微镜检查、荧光染色、印迹细胞学检查和眼前节光学相干断层扫描（AS - OCT）进行检查。在30天和120天时对兔实施安乐死，并对其角膜进行组织学和免疫组织化学处理。结果，实验组的兔角膜上皮恢复且透明，无上皮缺损。此外，角膜上皮中央区无杯状细胞。总之，我们的新治疗方法改善了角膜表面，是治疗兔LSCD的有效方法。
