School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia.
Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, VIC 3010, Australia.
Int J Mol Sci. 2024 Jan 16;25(2):1076. doi: 10.3390/ijms25021076.
In this study, novel solid lipid particles containing the adjuvant lipid monophosphoryl lipid A (termed 'SLN-A') were synthesised. The SLN-A particles were able to efficiently bind and form complexes with a DNA vaccine encoding the urease alpha subunit of . The resultant nanoparticles were termed lipoplex-A. In a mouse model of infection, the lipoplex-A nanoparticles were used to immunise mice, and the resultant immune responses were analysed. It was found that the lipoplex-A vaccine was able to induce high levels of antigen-specific antibodies and an influx of gastric CD4 T cells in vaccinated mice. In particular, a prime with lipoplex-A and a boost with soluble UreA protein induced significantly high levels of the IgG1 antibody, whereas two doses of lipoplex-A induced high levels of the IgG2c antibody. In this study, lipoplex-A vaccination did not lead to a significant reduction in colonisation in a challenge model; however, these results point to the utility of the system for delivering DNA vaccine-encoded antigens to induce immune responses and suggest the ability to tailor those responses.
在这项研究中，合成了含有佐剂脂质单磷酰脂质 A（称为“SLN-A”）的新型固体脂质颗粒。这些 SLN-A 颗粒能够有效地与编码 尿素酶 α 亚单位的 DNA 疫苗结合并形成复合物。所得的纳米颗粒称为脂质体 A。在 感染的小鼠模型中，使用脂质体 A 纳米颗粒对小鼠进行免疫，并分析了产生的免疫反应。结果发现，脂质体 A 疫苗能够诱导接种小鼠产生高水平的抗原特异性抗体和胃 CD4 T 细胞涌入。特别是，脂质体 A 进行初免和可溶性 UreA 蛋白进行加强免疫可诱导高水平的 IgG1 抗体，而两剂脂质体 A 可诱导高水平的 IgG2c 抗体。在这项研究中，脂质体 A 疫苗接种并没有在挑战模型中导致定植的显著减少；然而，这些结果表明该系统用于传递 DNA 疫苗编码抗原以诱导免疫反应的有效性，并表明能够调整这些反应。
