Jakobsen A, Bichel F, Sell A
Urol Res. 1979 Jun 22;7(2):109-12. doi: 10.1007/BF00254690.
The DNA distribution has been investigated in biopsies from human bladder carcinomas by means of flow cytometry (FCM). The FCM analysis was based on a preparation method yielding a suspension of single cell nuclei. Biopsies from 45 patients were analysed. The results demonstrate a correlation between the degree of cellular anaplasia and the occurrence of cell populations with increased DNA content in the tumours. Cells with diploid DNA content constituted more than 90% of well-differentiated tumours and normal bladder mucosa. Cell populations with aneuploid DNA content occurred in almost all biopsies with cellular anaplasia estimated as grades II and III by microscopic examination. The results indicate that FCM analysis may be a valuable adjunct to histology. The possible prognostic significance of altered DNA distribution in bladder tumours is discussed.
已通过流式细胞术（FCM）对人膀胱癌活检组织中的DNA分布进行了研究。FCM分析基于一种制备单细胞核悬液的方法。对45例患者的活检组织进行了分析。结果表明，肿瘤细胞的间变程度与DNA含量增加的细胞群体的出现之间存在相关性。DNA含量为二倍体的细胞在高分化肿瘤和正常膀胱黏膜中占比超过90%。通过显微镜检查估计为II级和III级细胞间变的几乎所有活检组织中都出现了非整倍体DNA含量的细胞群体。结果表明，FCM分析可能是组织学的一种有价值的辅助手段。文中还讨论了膀胱肿瘤中DNA分布改变可能具有的预后意义。
