Bichel P, Frederiksen P, Kjaer T, Thommesen P, Vindelov L L
Cancer. 1977 Sep;40(3):1206-11. doi: 10.1002/1097-0142(197709)40:3<1206::aid-cncr2820400334>3.0.co;2-z.
Flow microfluorometry (FMF) and transrectal fine-needle biopsy were used for obtaining DNA histograms from 50 patients suffering from various prostatic lesions. Based on the cytomorphological pattern, the material was classified as benign prostatic hyperplasia (29 cases), suspected carcinoma (1 case), well-differentiated carcinoma (6 cases), moderately differentiated carcinoma (12 cases) and poorly differentiated carcinoma (2 cases). The biopsy material was prepared for FMF analysis according to a new detergent technique. It was observed that increasing anaplasia paralleled an increasing occurrence of cell populations in the tetraploid and octoploid DNA region. According to the DNA histograms the moderately differentiated carcinomas could be divided into two groups: one with no or a few tetraploid cells (similar to the well-differentiated carcinomas), and another with a high percentage of tetraploid and octoploid cells (similar to the poorly differentiated carcinomas). FMF analysis in combination with fine-needle biopsy is therefore proposed as a valuable addition to the cytomorphological classification of human prostatic carcinoma.
采用流式细胞荧光测定法（FMF）和经直肠细针穿刺活检，从50例患有各种前列腺病变的患者中获取DNA直方图。根据细胞形态学模式，将材料分为良性前列腺增生（29例）、疑似癌（1例）、高分化癌（6例）、中分化癌（12例）和低分化癌（2例）。活检材料根据一种新的去污剂技术制备用于FMF分析。观察到间变增加与四倍体和八倍体DNA区域细胞群出现增加平行。根据DNA直方图，中分化癌可分为两组：一组无或仅有少量四倍体细胞（类似于高分化癌），另一组四倍体和八倍体细胞百分比高（类似于低分化癌）。因此，建议将FMF分析与细针穿刺活检相结合，作为对人类前列腺癌细胞形态学分类的有价值补充。
