Hassan Mervat G, Elmezain Waleed A, Baraka Dina M, AboElmaaty Sabah A, Elhassanein Ahmed, Ibrahim Riyad Mohammed, Hamed Ahmed A
Botany and Microbiology Department, Faculty of Science, Benha University, Benha 33516, Egypt.
Department of Mathematics, College of Science, University of Bisha, P.O. Box 551, Bisha 61922, Saudi Arabia.
Microorganisms. 2024 Jan 8;12(1):127. doi: 10.3390/microorganisms12010127.
Cancer is a huge global disease burden. Every year, tens of millions of people worldwide are diagnosed with cancer, and more than half of them die as a result of it. The great biodiversity of the marine environment has increasingly piqued the interest of experts, especially in the field of drug discovery. The marine fungus WA7S6 has been selected among a group of fungi isolated from marine sponges as it exhibits a pronounced antimicrobial activity toward a group of pathogenic microbes. The fungus has been identified genetically by amplification and analysis of its 18srRNA gene. The fungus crude extract has been obtained by cultivation of the fungus on rice media. The crude extract was tested for antibacterial activity against a variety of pathogenic microorganisms. The results demonstrated a pronounced antimicrobial action against , , , and . Furthermore, we tested the antioxidant potential of the Aspergillus fumigatus WA7S6 crude extract using three different methods: ATBS, DPPH, and lipid peroxidation assays. Results showed that the crude extract WA7S6 had an IC50 value of 21.35 µg/mL. The anticancer potential of the crude extract was also evaluated against cancer cell lines such as Hela, MCF, and WI-38. The chemical profiling of the fungus extract was identified via GC-mass and in silico molecular docking of the identified compounds on heme oxygenase, as a stress protein included in cellular protection, antioxidant, and anti-inflammatory activities, suggesting that some compounds, such as 9-Tetradecynoic acid, 11-Hexadecynoic acid, methyl ester, and dehydromevalonic lactone, could be relevant for antioxidant purposes.
癌症是一个巨大的全球疾病负担。每年，全球数千万人被诊断出患有癌症，其中超过一半的人因此死亡。海洋环境的巨大生物多样性越来越引起专家们的兴趣，尤其是在药物发现领域。海洋真菌WA7S6是从一组从海洋海绵中分离出的真菌中挑选出来的，因为它对一组致病微生物表现出显著的抗菌活性。通过对其18srRNA基因的扩增和分析，从基因上鉴定了该真菌。通过在大米培养基上培养该真菌获得了真菌粗提物。对粗提物针对多种致病微生物的抗菌活性进行了测试。结果表明，它对[具体微生物名称1]、[具体微生物名称2]、[具体微生物名称3]和[具体微生物名称4]具有显著的抗菌作用。此外，我们使用三种不同的方法：ABTS、DPPH和脂质过氧化测定法，测试了烟曲霉WA7S6粗提物的抗氧化潜力。结果表明，粗提物WA7S6的IC50值为21.35 µg/mL。还针对Hela、MCF和WI-38等癌细胞系评估了粗提物的抗癌潜力。通过气相色谱 - 质谱联用（GC - mass）对真菌提取物进行化学分析，并对鉴定出的化合物与作为细胞保护、抗氧化和抗炎活性中包含的应激蛋白的血红素加氧酶进行计算机模拟分子对接，表明一些化合物，如9 - 十四碳炔酸、11 - 十六碳炔酸甲酯和脱氢甲羟戊酸内酯，可能与抗氧化目的相关。
