Sasaki K
Hokkaido Igaku Zasshi. 1984 Nov;59(6):679-89.
ABH isoantigen of 154 superficial urothelial tumor including 11 carcinoma in situ of the bladder (CIS) was investigated by the avidin-biotin-peroxidase complex (ABC) method, and the results were compared with those obtained by the specific red cell adherence (SRCA) test. T (Thomsen-Friedenreich) antigen, a precursor of other blood group antigen than ABC, was also investigated by ABC method. ABH antigen detected by ABC method seemed to be correlated to tumor grade and recurrence rate, while that by SRCA method did not. Sixty two percent of the low grade papillary bladder tumor of blood group other than O was positive by ABC method. As for the recurrence rate of low grade tumor, 11 of 33 cases (33%) with positive ABH isoantigen showed recurrence, while 13 of 21 cases (62%) without ABH isoantigen did so. In CIS, ABH antigen was deleted in 82 percent using ABC method. ABC method is more sensitive and more specific than SRCA, and the sensitivity was increased in blood O. T antigen, which is expressed in many carcinomas, (defined T(+) by Coon) is usually not detected in normal epithelium. Normally, T antigen is cryptic but can be unmasked with sialidase (cryptic T(+)). The cells which lacked T even after sialidase treatment are called cryptic T(-). We investigated T antigen expression in CIS lesion and ureteropelvic tumor by using T specific lectin (peanut agglutinin). In nine cases of CIS which ABH was negative, cryptic T(+), T(+) and cryptic T(-) were found in three, one and five cases, respectively. In ten cases of high grade ureteropelvic tumor, which ABH was all negative, cryptic T(+) was found in five cases. Of these five cases, three are well over five years after surgery. These data, although preliminary, indicate that, by combining two markers (ABH and T antigen), prognosis of urothelial tumor may be predicted better.
采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法对154例浅表性尿路上皮肿瘤（包括11例膀胱原位癌，CIS）的ABH同种抗原进行了研究，并将结果与特异性红细胞黏附（SRCA）试验的结果进行了比较。还采用ABC法研究了T（汤姆森-弗里德赖希）抗原，它是除ABC之外其他血型抗原的前体。ABC法检测到的ABH抗原似乎与肿瘤分级和复发率相关，而SRCA法检测的结果则不然。ABC法检测显示，62%的非O血型低级别乳头状膀胱肿瘤呈阳性。至于低级别肿瘤的复发率，ABH同种抗原阳性的33例病例中有11例（33%）复发，而ABH同种抗原阴性的21例病例中有13例（62%）复发。在CIS中，采用ABC法检测发现82%的病例ABH抗原缺失。ABC法比SRCA法更敏感、更特异，且在O血型中敏感性更高。T抗原在许多癌中表达（库恩定义为T(+)），正常上皮中通常检测不到。正常情况下，T抗原是隐蔽的，但可被唾液酸酶暴露（隐蔽性T(+)）。即使经过唾液酸酶处理仍缺乏T抗原的细胞称为隐蔽性T(-)。我们使用T特异性凝集素（花生凝集素）研究了CIS病变和输尿管肾盂肿瘤中T抗原的表达。在9例ABH阴性的CIS病例中，分别有3例、1例和5例发现隐蔽性T(+)、T(+)和隐蔽性T(-)。在10例高级别输尿管肾盂肿瘤病例中，ABH均为阴性，其中5例发现隐蔽性T(+)。在这5例病例中，有3例术后已超过5年。这些数据虽然是初步的，但表明通过结合两种标志物（ABH和T抗原），可能能更好地预测尿路上皮肿瘤的预后。
