Chai Jianmin, Liu Zeyue, Wu Jun, Kang Yuan, Abdelsattar Mahmoud M, Zhao Wei, Wang Shiqin, Yang Shuli, Deng Feilong, Li Ying, Zhuang Yimin, Zhang Naifeng
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, China.
Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2024 Jan 8;14:1296116. doi: 10.3389/fmicb.2023.1296116. eCollection 2023.
The ketone body β-hydroxybutyric acid (BHB) plays critical roles in cellular proliferation and metabolic fuel utilization; however, its effects on the rumen microbiota remain unknown.
Here, three doses of BHB (low, medium, and high) were supplemented to early-weaned goat kids.
Compared with controls, the beneficial effects of BHB on growth and rumen development were observed in goats at 90 days of age (d). The low dose of dietary BHB increased the concentration of rumen acetate, propionate, and butyrate on d90. The sequencing results of the rumen microbiota revealed marked shifts in rumen microbial community structure after early-weaned goat kids consumed BHB for 2 months. The signature bacterial ASVs for each treatment were identified and were the main drivers contributing to microbial interactions in the rumen. The bacteria associated with rumen weight were also correlated with body weight. Some classified bacterial signatures, including , and , were related to rumen volatile fatty acids and host development.
Overall, dietary BHB altered rumen microbiota and environments in young goats, which contributed to rumen development and growth.
酮体β-羟基丁酸（BHB）在细胞增殖和代谢燃料利用中起关键作用；然而，其对瘤胃微生物群的影响尚不清楚。
在此，向早期断奶的山羊幼崽补充三种剂量的BHB（低、中、高）。
与对照组相比，在90日龄（d）的山羊中观察到BHB对生长和瘤胃发育的有益影响。低剂量的日粮BHB在第90天增加了瘤胃乙酸盐、丙酸盐和丁酸盐的浓度。瘤胃微生物群的测序结果显示，早期断奶的山羊幼崽食用BHB 2个月后，瘤胃微生物群落结构发生了显著变化。确定了每种处理的标志性细菌扩增子序列变异（ASVs），它们是瘤胃中微生物相互作用的主要驱动因素。与瘤胃重量相关的细菌也与体重相关。一些分类的细菌特征，包括 、 和 ，与瘤胃挥发性脂肪酸和宿主发育有关。
总体而言，日粮BHB改变了幼山羊的瘤胃微生物群和环境，这有助于瘤胃发育和生长。
