College of Ecological Engineering, Guizhou University of Engineering Science, Bijie City, 551700, Guizhou Province, China.
Guizhou Province Key Laboratory of Ecological Protection and Restoration of Typical Plateau Wetlands, Bijie City, 551700, Guizhou Province, China.
Microb Ecol. 2024 Jan 24;87(1):36. doi: 10.1007/s00248-024-02345-8.
Clarifying the response of soil microbial communities to vegetation restoration is essential to comprehend biogeochemical processes and ensure the long-term viability of forest development. To assess the variations in soil microbial communities throughout the growth of Pinus armandii plantations in the karst region, we utilized the "space instead of time" approach and selected four P. armandii stands with ages ranging from 10 to 47 years, along with a grassland control. The microbial community structure was determined by conducting Illumina sequencing of the 16 S rRNA gene and the ITS gene, respectively. The results demonstrated that afforestation with P. armandii significantly influenced soil microbial communities, as indicated by notable differences in bacterial and fungal composition and diversity between the plantations and the control. However, soil microbe diversity did not display significant variation across stand ages. Moreover, the bacterial community exhibited higher responsiveness to age gradients compared to the fungal community. Soil physicochemical factors play a critical role in elucidating microbial diversity and community composition variations during restoration processes. TN, AN, TP, AP, SOC, AK, and pH were the most significant influencing factors for the composition of bacterial community, while TC, SOC, pH, and TC were the most significant influencing factors for the composition of fungal community. Our findings indicate substantial changes in soil bacterial and fungal communities across successive stages of development. Additionally, the changes in dominant bacteria and fungi characteristics across the age gradient were primarily attributed to variations in the prevailing soil conditions and chemical factors.
阐明土壤微生物群落对植被恢复的响应对于理解生物地球化学过程和确保森林发展的长期可行性至关重要。为了评估喀斯特地区华山松人工林生长过程中土壤微生物群落的变化，我们采用了“空间代替时间”的方法，选择了 4 个年龄分别为 10 年、20 年、30 年和 47 年的华山松人工林和一个草地对照。通过对 16S rRNA 基因和 ITS 基因进行 Illumina 测序，分别确定了微生物群落结构。结果表明，华山松造林显著影响了土壤微生物群落，人工林与对照之间细菌和真菌组成和多样性存在显著差异。然而，林分年龄之间的土壤微生物多样性没有显著变化。此外，细菌群落对年龄梯度的响应性高于真菌群落。土壤理化因子在阐明恢复过程中微生物多样性和群落组成变化方面起着关键作用。TN、AN、TP、AP、SOC、AK 和 pH 是细菌群落组成的最显著影响因素，而 TC、SOC、pH 和 TC 是真菌群落组成的最显著影响因素。我们的研究结果表明，土壤细菌和真菌群落在连续发育阶段发生了显著变化。此外，年龄梯度上优势细菌和真菌特征的变化主要归因于土壤条件和化学因素的变化。
