Bessler W G, Henning U
Z Immunitatsforsch Immunobiol. 1979 Jun;155(5):387-98.
Protein I from the outer membrane of Escherichia coli is a B-lymphocyte mitogen in mice. Polyclonal activation of mouse splenocytes was demonstrated by 3H-thymidine incorporation into DNA, 3H-uridine incorporation into RNA, and by a hemolytic plaque assay in three inbred mouse strains. B-lymphocytes from LPS responder mice (C57Bl/10, STU/nu/nu) and LPS non-responder mice (C3H/HeJ) both responded well to protein I. The presence of serum was not necessary for mitogenicity; bovine serum albumin exhibited a beneficial effect on serum-depleted cultures. Thymocytes of C3H/HeJ mice were not activated by protein I. Protein II* from E. coli was also tested in our systems and showed a weak B-lymphocyte stimulatory activity. Human peripheral blood lymphocytes were not activated.
来自大肠杆菌外膜的蛋白质I是小鼠中的一种B淋巴细胞有丝分裂原。通过将3H-胸腺嘧啶核苷掺入DNA、3H-尿苷掺入RNA以及在三种近交小鼠品系中进行溶血空斑试验，证实了小鼠脾细胞的多克隆激活。来自LPS反应性小鼠（C57Bl/10、STU/nu/nu）和LPS无反应性小鼠（C3H/HeJ）的B淋巴细胞对蛋白质I均有良好反应。有丝分裂原性并不需要血清的存在；牛血清白蛋白对血清耗尽的培养物有有益作用。C3H/HeJ小鼠的胸腺细胞未被蛋白质I激活。来自大肠杆菌的蛋白质II*也在我们的系统中进行了测试，显示出较弱的B淋巴细胞刺激活性。人外周血淋巴细胞未被激活。
