Jiao Yang, Wang Xin, Yu Aizhen, Wu Li, Li Hongping
Kashgar People's Hospital, Kashgar Prefecture, Xinjiang Uygur Autonomous Region, Kashgar, China.
Neonatology Department, Affiliated Shenzhen Children's Hospital of Shantou University Medical College, Shenzhen, Guangdong, China.
Front Pediatr. 2024 Nov 28;12:1467581. doi: 10.3389/fped.2024.1467581. eCollection 2024.
Facilitating the development of the sucking function in early stages of preterm infants holds substantial potential for influencing their long-term outcomes. To this end, our team has devised a sucking-rewarded automatic feeding device specifically tailored for preterm infants. The present study is designed to investigate the impacts of this innovative device, utilizing a multi-omics profiling approach, on beagle dogs as a surrogate model.
This study involved seven-day-old male newborn beagle puppies, carefully selected and matched in terms of body weights. The participants were stratified into two groups: the experimental group (AFG, sucking-rewarded feeding group) and the control group (PFG). After a 14-day intervention period, fecal and blood samples were systematically collected from each dog. The collected samples were then subjected to distinct profiling analyses, encompassing the assessment of gut microbial composition, plasma metabolic profiles, and proteomic expression profiles.
The gut microbial data showed a significant difference between the AFG and PFG groups based on Bray-Curtis dissimilarity ( = 0.048), and the relative abundance of was significantly more abundant in the AFG group compared to the PFG group. The significantly different metabolites between the two groups were enriched in functional metabolic pathways related to amino acids, fatty acid metabolism, and the nervous system. Notably, neurotransmitter L-glutamic acid was significantly up-regulated in the AFG group. Moreover, the significantly different proteins between the two groups were enriched in GO terms related to oxygen transport, oxygen binding, iron ion binding, hemoglobin complex, and heme binding. Among them, proteins A0A8C0MTD2, P60524, P60529 were significantly up-regulated in the AFG group. Notably, , L-glutamic acid, A0A8C0MTD2, P60524, and P60529 were correlated with each other through correlation analysis, these molecules play important roles in the neural function and neurodevelopment.
Our investigation elucidated discernible modifications in gut microbial composition, plasma metabolic profiles, and proteomic expression patterns in beagle dogs subjected to the sucking-rewarded automatic feeding device.
促进早产儿早期吸吮功能的发展对其长期预后具有重大潜在影响。为此，我们团队设计了一种专门为早产儿量身定制的吸吮奖励自动喂养装置。本研究旨在利用多组学分析方法，以比格犬作为替代模型，研究这种创新装置的影响。
本研究涉及7日龄雄性新生比格犬幼犬，根据体重进行精心挑选和匹配。参与者被分为两组：实验组（AFG，吸吮奖励喂养组）和对照组（PFG）。经过14天的干预期后，从每只狗身上系统地采集粪便和血液样本。然后对收集的样本进行不同的分析，包括肠道微生物组成评估、血浆代谢谱分析和蛋白质组表达谱分析。
基于Bray-Curtis差异度（=0.048），肠道微生物数据显示AFG组和PFG组之间存在显著差异，与PFG组相比，AFG组中 的相对丰度显著更高。两组之间显著不同的代谢物在与氨基酸、脂肪酸代谢和神经系统相关的功能代谢途径中富集。值得注意的是，神经递质L-谷氨酸在AFG组中显著上调。此外，两组之间显著不同 的蛋白质在与氧气运输、氧气结合、铁离子结合、血红蛋白复合物和血红素结合相关的基因本体（GO）术语中富集。其中，蛋白质A0A8C0MTD2、P60524、P60529在AFG组中显著上调。值得注意的是，通过相关性分析， 、L-谷氨酸、 A0A8C0MTD2、P60524和P60529相互关联，这些分子在神经功能和神经发育中起重要作用。
我们的研究阐明了接受吸吮奖励自动喂养装置的比格犬在肠道微生物组成、血浆代谢谱和蛋白质组表达模式方面有明显变化。
