Faculty of Biology Medicine and Health, School of Biological Sciences, Division of Neuroscience, The University of Manchester, Manchester, UK.
Geoffrey Jefferson Brain Research Centre, Manchester Academic Health Science Centre (MAHSC), Manchester, UK.
Brain Pathol. 2024 Sep;34(5):e13243. doi: 10.1111/bpa.13243. Epub 2024 Jan 25.
Formalin-fixed paraffin-embedded (FFPE) brain tissue held in tissue banks constitutes a valuable research resource, especially when associated with clinical annotations and longitudinal psychometric testing. Apolipoprotein-E (APOE) genotyping is important to fully characterise this resource, however older FFPE tissue may not be suitable for genotyping. We performed polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays on DNA extracted from post-mortem FFPE brain tissue ranging from 2-19 years old. A maximum of three years in paraffin was determined for robust APOE genotyping of FFPE tissue using PCR-RFLP which may suggest prolonged storage of fixed tissue as FFPE blocks may have deleterious effects on DNA.
福尔马林固定石蜡包埋（FFPE）脑组织存放在组织库中，构成了有价值的研究资源，特别是当与临床注释和纵向心理测试相关联时。载脂蛋白 E（APOE）基因分型对于充分描述这种资源非常重要，然而，较老的 FFPE 组织可能不适合基因分型。我们对从死后 FFPE 脑组织中提取的 DNA 进行了聚合酶链反应限制性片段长度多态性（PCR-RFLP）检测，这些组织的保存时间为 2-19 年。通过 PCR-RFLP 对 FFPE 组织进行强有力的 APOE 基因分型的最佳时间是在石蜡中保存最多三年，这可能表明固定组织的长期保存作为 FFPE 块可能对 DNA 有有害影响。
