Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Sci Rep. 2023 Jun 22;13(1):10188. doi: 10.1038/s41598-023-37287-7.
Green nanotechnology has recently been recognized as a more proper and safer tool for medical applications thanks to its natural reductions with low toxicity and avoidance of injurious chemicals. The macroalgal biomass was used for nanocellulose biosynthesis. Algae are abundant in the environment and have a high content of cellulose. In our study, we extracted parent cellulose from Ulva lactuca where consecutive treatments extracted cellulose to obtain an insoluble fraction rich in cellulose. The extracted cellulose has the same results obtained by matching it with reference cellulose, especially the same Fourier transform infrared (FTIR) and X-Ray diffraction (XRD) analysis peaks. Nanocellulose was synthesized from extracted cellulose with hydrolysis by sulfuric acid. Nanocellulose was examined by Scanning electron microscope (SEM) shown by a slab-like region as Fig. 4a and Energy dispersive X-ray (EDX) to examine the chemical composition. The size of nanocellulose in the range of 50 nm is calculated by XRD analysis. Antibacterial examination of nanocellulose was tested against Gram+ bacteria like Staphylococcus aureus (ATCC6538), Klebsiella pneumonia (ST627), and Gram-negative bacteria such as Escherichia coli (ATCC25922), and coagulase-negative Staphylococci (CoNS) to give 4.06, 4.66, 4.93 and 4.43 cm as respectively. Comparing the antibacterial effect of nanocellulose with some antibiotics and estimating minimal Inhibitory Concentration (MIC) of nanocellulose. We tested the influence of cellulose and nanocellulose on some fungi such as Aspergillus flavus, Candida albicans, and Candida tropicalis. These results demonstrate that nanocellulose could be developed as an excellent solution to these challenges, making nanocellulose extracted from natural algae a very important medical material that is compatible with sustainable development.
绿色纳米技术最近被认为是一种更合适和更安全的医学应用工具，因为它具有天然的低毒性和避免有害化学物质的减少。宏藻类生物质被用于纳米纤维素的生物合成。藻类在环境中丰富，纤维素含量高。在我们的研究中，我们从绿藻中提取了原纤维素，经过连续处理提取纤维素，得到了富含纤维素的不溶性部分。提取的纤维素与参考纤维素相匹配，结果相同，特别是傅里叶变换红外（FTIR）和 X 射线衍射（XRD）分析峰相同。纳米纤维素是通过硫酸水解从提取的纤维素中合成的。纳米纤维素通过扫描电子显微镜（SEM）进行检查，如图 4a 所示，呈现出板状区域，并用能量色散 X 射线（EDX）检查其化学成分。通过 XRD 分析计算出纳米纤维素的尺寸在 50nm 范围内。纳米纤维素的抗菌试验是针对革兰氏阳性菌，如金黄色葡萄球菌（ATCC6538）、肺炎克雷伯菌（ST627），和革兰氏阴性菌，如大肠杆菌（ATCC25922）和凝固酶阴性葡萄球菌（CoNS）进行的，结果分别为 4.06、4.66、4.93 和 4.43cm。将纳米纤维素的抗菌效果与一些抗生素进行比较，并估计纳米纤维素的最小抑菌浓度（MIC）。我们测试了纤维素和纳米纤维素对一些真菌的影响，如黄曲霉、白色念珠菌和热带念珠菌。这些结果表明，纳米纤维素可以作为解决这些问题的一种极好的解决方案，使从天然藻类中提取的纳米纤维素成为一种非常重要的与可持续发展兼容的医用材料。
