Burdík Martin, Kužela Tomáš, Fojtů Dušan, Elisek Petr, Hrnčiřík Josef, Jašek Roman, Ingr Marek
Department of Informatics and Artificial Intelligence, Faculty of Applied Informatics, Tomas Bata University in Zlín, Nad Stráněmi 4511, 760 05 Zlín, Czech Republic.
Department of Physics and Materials Engineering, Faculty of Technology, Tomas Bata University in Zlín, Nám. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic.
Sensors (Basel). 2024 Jan 19;24(2):643. doi: 10.3390/s24020643.
Optical tweezers (OT), or optical traps, are a device for manipulating microscopic objects through a focused laser beam. They are used in various fields of physical and biophysical chemistry to identify the interactions between individual molecules and measure single-molecule forces. In this work, we describe the development of a homemade optical tweezers device based on a cost-effective IR diode laser, the hardware, and, in particular, the software controlling it. It allows us to control the instrument, calibrate it, and record and process the measured data. It includes the user interface design, peripherals control, recording, A/D conversion of the detector signals, evaluation of the calibration constants, and visualization of the results. Particular stress is put on the signal filtration from noise, where several methods were tested. The calibration experiments indicate a good sensitivity of the instrument that is thus ready to be used for various single-molecule measurements.
光镊（OT），即光学捕获装置，是一种通过聚焦激光束来操纵微观物体的设备。它们被用于物理和生物物理化学的各个领域，以识别单个分子之间的相互作用并测量单分子力。在这项工作中，我们描述了一种基于经济高效的红外二极管激光器的自制光镊装置的开发，包括硬件，特别是控制它的软件。它使我们能够控制仪器、对其进行校准，并记录和处理测量数据。它包括用户界面设计、外围设备控制、记录、探测器信号的模数转换、校准常数的评估以及结果的可视化。特别强调了从噪声中进行信号过滤，其中测试了几种方法。校准实验表明该仪器具有良好的灵敏度，因此已准备好用于各种单分子测量。
