Liu Hao, Chen Yawen, Wang Heyu, Huang Yaxuan, Hu Ying, Zhao Yuxiang, Gong Yifu
Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315200, China.
College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315200, China.
Mar Drugs. 2023 Dec 20;22(1):7. doi: 10.3390/md22010007.
Fucoxanthin, a vital secondary metabolite produced by marine diatoms, has great economic value and research potential. However, its popularization and application have been greatly restricted due to its low content, difficult extraction, and high production cost. Methyl jasmonic acid (MeJA) exerts similar inductive hormones in the growth and development as well as metabolic processes of plants. In (), MeJA treatment can increase fucoxanthin content. In this study, the effects of different concentrations of MeJA on the cell growth and the fucoxanthin content of were explored. Meanwhile, this study used high-throughput sequencing technology for transcriptome sequencing of and subsequently performed differential gene expression analysis, gene ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and weighted gene co-expression network analysis (WGCNA) for screening the hub genes for the promotion of fucoxanthin synthesis with MeJA-treated . On this basis, the functions of the hub genes for the promotion of fucoxanthin synthesis with MeJA-treated were further analyzed. The results revealed that the carotenoid synthesis-related genes PHATRDRAFT_54800 and PHATRDRAFT_20677 were the hub genes for the promotion of fucoxanthin synthesis with MeJA-treated . PHATRDRAFT_54800 may be a carotenoid isomerase, while PHATRDRAFT_20677 may be involved in the MeJA-stimulated synthesis of fucoxanthin by exerting the role of SDR family NAD(P)-dependent oxidoreductases.
岩藻黄质是海洋硅藻产生的一种重要次生代谢物，具有巨大的经济价值和研究潜力。然而，由于其含量低、提取困难和生产成本高，其推广应用受到很大限制。茉莉酸甲酯（MeJA）在植物的生长发育以及代谢过程中发挥着类似的诱导激素作用。在（）中，MeJA处理可增加岩藻黄质含量。本研究探讨了不同浓度的MeJA对（某植物，原文未明确）细胞生长和岩藻黄质含量的影响。同时，本研究利用高通量测序技术对（某植物，原文未明确）进行转录组测序，随后进行差异基因表达分析、基因本体（GO）富集分析、京都基因与基因组百科全书（KEGG）富集分析以及加权基因共表达网络分析（WGCNA），以筛选出用MeJA处理的（某植物，原文未明确）促进岩藻黄质合成的关键基因。在此基础上，进一步分析了用MeJA处理的（某植物，原文未明确）促进岩藻黄质合成的关键基因的功能。结果表明，类胡萝卜素合成相关基因PHATRDRAFT_54800和PHATRDRAFT_20677是用MeJA处理的（某植物，原文未明确）促进岩藻黄质合成的关键基因。PHATRDRAFT_54800可能是一种类胡萝卜素异构酶，而PHATRDRAFT_20677可能通过发挥SDR家族NAD(P)依赖性氧化还原酶的作用参与MeJA刺激的岩藻黄质合成。
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