猫肝细胞中的蛋白质降解
Protein degradation in cat liver cells.
作者信息
Silva S V, Mercer J R
出版信息
Biochem J. 1986 Dec 15;240(3):843-6. doi: 10.1042/bj2400843.
Body proteins in cats were prelabelled with [14C]valine, and protein degradation was studied in isolated hepatocytes. Amino acids appeared to have a direct inhibitory effect on protein degradation, but the effects were generally smaller than those previously shown in the rat. The amino acid control of protein degradation in the cat differs from that in the rat, as shown by the lack of effects of glutamine, asparagine, arginine or methionine in cat hepatocytes. This may be related to the unique features of protein metabolism of this species. NH4Cl, leupeptin and amino acids, which suppress lysosomal protein degradation by different mechanisms, caused less than 30% inhibition of protein degradation when used at the optimum concentrations reported for the rat. The ability of the lysosomal system to respond to nutritional deprivation is apparently lower in the cat than in the rat.
用[14C]缬氨酸对猫的身体蛋白质进行预标记，然后在分离的肝细胞中研究蛋白质降解。氨基酸似乎对蛋白质降解有直接抑制作用，但这种作用通常比之前在大鼠中显示的要小。猫肝细胞中蛋白质降解的氨基酸调控与大鼠不同，如谷氨酰胺、天冬酰胺、精氨酸或蛋氨酸对猫肝细胞没有影响所示。这可能与该物种蛋白质代谢的独特特征有关。NH4Cl、亮抑蛋白酶肽和氨基酸通过不同机制抑制溶酶体蛋白质降解，当以报道的大鼠最佳浓度使用时，对蛋白质降解的抑制作用小于30%。猫的溶酶体系统对营养缺乏的反应能力显然低于大鼠。
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