氨基酸、氨和亮抑蛋白酶肽对分离的大鼠肝细胞中蛋白质合成与降解的影响。
Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes.
作者信息
Seglen P O
出版信息
Biochem J. 1978 Aug 15;174(2):469-74. doi: 10.1042/bj1740469.
Abstract
Protein synthesis in isolated rat hepatocytes, as measured by the incorporation of [14C]-valine at constant specific radioactivity, proceeded at a rate of 0.3-0.5%/h in an unsupplemented medium, i.e. only about one-tenth the rate of protein degradation (4%/h). Leupeptin, which inhibits lysosomal protein degradation (previously found to be 75% of the total degradation in hepatocytes), had no effect on protein synthesis, showing that endogenous protein degradation supplied amino acids in excess of the substrate requirements for protein synthesis. The inhibition of protein synthesis by NH4Cl (another inhibitor of lysosomal protein degradation) as well as the stimulation by a physiological amino acid mixture must therefore represent indirect effects, either on general energy metabolism, or on unknown regulatory processes.
摘要
通过在恒定比放射性下掺入[14C]-缬氨酸来测定，分离的大鼠肝细胞中的蛋白质合成在未补充培养基中以0.3 - 0.5%/小时的速率进行，即仅约为蛋白质降解速率（4%/小时）的十分之一。亮抑蛋白酶肽可抑制溶酶体蛋白降解（先前发现其占肝细胞总降解的75%），但对蛋白质合成无影响，这表明内源性蛋白质降解提供的氨基酸超过了蛋白质合成的底物需求。因此，NH4Cl（另一种溶酶体蛋白降解抑制剂）对蛋白质合成的抑制以及生理氨基酸混合物的刺激必定代表了对一般能量代谢或未知调节过程的间接影响。
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