Rowe E S
Biochemistry. 1987 Jan 13;26(1):46-51. doi: 10.1021/bi00375a007.
It has previously been shown that alcohol has different effects on the gel to liquid-crystal phase transition of phosphatidylcholines (PC's) and phosphatidylethanolamines (PE's) [Rowe, E. S. (1985) Biochim. Biophys. Acta 813, 321-330]. In this investigation, the thermotropic properties of binary PE-PC mixtures were studied in the presence of ethanol in order to determine whether the differential interactions of alcohol with PC and PE would lead to lateral phase separations. Phase diagrams of the dilaurylphosphatidylethanolamine-dipalmitoylphosphatidylcholine [PE(12:0)-PC(16:0)] system were constructed in the presence and absence of ethanol. It was shown that lateral phase separations occur in the gel phase over a certain composition range in the presence of 100 mg/mL ethanol. In the absence of alcohol these two lipids are miscible in both the gel and liquid-crystal states. The data suggest that in the presence of ethanol these lateral phase separations involve the coexistence of regular bilayer gel and the fully interdigitated gel phase, which has previously been shown to occur in pure PC(16:0) under these conditions [Simon, S. A., & McIntosh, T. J. (1984) Biochim. Biophys. Acta 773, 169-172]. The biological implications of these findings are discussed.
先前的研究表明，酒精对磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）从凝胶相到液晶相的转变有不同影响[罗伊，E.S.（1985年）《生物化学与生物物理学报》813卷，321 - 330页]。在本研究中，为了确定酒精与PC和PE的差异相互作用是否会导致横向相分离，研究了乙醇存在下二元PE - PC混合物的热致性质。在有和没有乙醇的情况下构建了二月桂酰磷脂酰乙醇胺 - 二棕榈酰磷脂酰胆碱[PE(12:0) - PC(16:0)]体系的相图。结果表明，在100 mg/mL乙醇存在下，凝胶相中在一定组成范围内会发生横向相分离。在没有酒精的情况下，这两种脂质在凝胶态和液晶态均互溶。数据表明，在乙醇存在下，这些横向相分离涉及规则双层凝胶和完全交错凝胶相的共存，此前已表明在这些条件下纯PC(16:0)中会出现这种情况[西蒙，S.A.，& 麦金托什，T.J.（1984年）《生物化学与生物物理学报》773卷，169 - 172页]。讨论了这些发现的生物学意义。
