Duan Jiajia, Wang Wei, Jiang Tao, Bai Xiaoyang, Liu Chuanxin
Department of Clinical Laboratory, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China.
Department of Neurology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
Front Microbiol. 2022 Nov 15;13:1046894. doi: 10.3389/fmicb.2022.1046894. eCollection 2022.
Depression is a heterogeneous mental disorder that has been linked to disturbances in the gut microbiome. As an essential part of the gut microbiome, gut virome may play critical roles in disease progression and development. However, the relationship between the effect of gut virome on neurotransmitter metabolism and depression is unknown. We evaluated the alterations of gut virome and neurotransmitters in chronic restraint stress (CRS)-induced mouse model of depression based on viral metagenomics and LC-MS/MS metabolomics analyses. The results reveal that the gut virome profile of CRS group differed significantly from CON group. was the most abundant differential viral family in both groups, followed by , while was only enriched in CRS group of the top 100 differential viruses. The differential viruses that predicted to phage, phage and phage were enriched in CRS group. Furthermore, 12 differential neurotransmitters primarily involved in the tryptophan metabolism pathway were altered in depressive-like mice. Besides, tryptamine and 5-methoxytryptamine hydrochloride were strongly associated with differential viruses belonging to and . Our findings provide new insight into understanding the potential role of the gut virome and metabolites in depression.
抑郁症是一种异质性精神障碍，已被发现与肠道微生物群紊乱有关。作为肠道微生物群的重要组成部分，肠道病毒组可能在疾病进展和发展中发挥关键作用。然而，肠道病毒组对神经递质代谢的影响与抑郁症之间的关系尚不清楚。我们基于病毒宏基因组学和液相色谱-串联质谱代谢组学分析，评估了慢性束缚应激（CRS）诱导的抑郁症小鼠模型中肠道病毒组和神经递质的变化。结果显示，CRS组的肠道病毒组图谱与对照组有显著差异。 是两组中最丰富的差异病毒家族，其次是 ，而 在差异最大前100种病毒中仅在CRS组富集。预测为 噬菌体、 噬菌体和 噬菌体的差异病毒在CRS组中富集。此外，在抑郁样小鼠中，12种主要参与色氨酸代谢途径的差异神经递质发生了改变。此外，色胺和盐酸5-甲氧基色胺与属于 和 的差异病毒密切相关。我们的研究结果为理解肠道病毒组和代谢产物在抑郁症中的潜在作用提供了新的见解。
