• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

运用RNA测序技术探究单次推拿干预对轻微慢性缩窄性损伤大鼠即时镇痛作用的机制

Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq.

作者信息

Wang Hourong, Liu Zhifeng, Yu Tianyuan, Zhang Yingqi, Xu Yajing, Jiao Yi, Guan Qian, Liu Di

机构信息

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.

Department of Tuina and Pain Management, Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing, China.

出版信息

Front Neurosci. 2022 Oct 4;16:1007432. doi: 10.3389/fnins.2022.1007432. eCollection 2022.

DOI:10.3389/fnins.2022.1007432
PMID:36267229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9577287/
Abstract

Previous studies have proved and investigated the mechanism of the analgesic effect of tuina treatment on neuropathic pain. The purpose of this study was to analyze changes in gene expression in the dorsal root ganglia (DRG) and spinal dorsal horn (SDH) after 1-time tuina intervention to investigate the immediate analgesic mechanism by tuina. An improvement in nociceptive behavior in minor chronic constriction injury (CCI) rats after 1-time tuina was observed. 1-time tuina was more effective in the amelioration of thermal hyperalgesia, but no changes were found in the ultrastructure of DRG and SDH. Sixty-five differentially expressed genes (DEGs) modulated by tuina were detected in the DRG and 123 DEGs were detected in the SDH. Potential immediate analgesic mechanisms of tuina were analyzed by the Kyoto Encyclopedia of Genes and Genomes. DEGs were enriched in 75 pathways in DRG, and 107 pathways in SDH. The immediate analgesic mechanism of tuina is related to the calcium signaling pathway, thermogenesis, and regulation of lipolysis in adipocytes.

摘要

以往的研究已经证实并探讨了推拿治疗神经性疼痛的镇痛作用机制。本研究的目的是分析单次推拿干预后背根神经节（DRG）和脊髓背角（SDH）中基因表达的变化，以探讨推拿的即时镇痛机制。观察到单次推拿后轻微慢性压迫损伤（CCI）大鼠的伤害性感受行为有所改善。单次推拿在改善热痛觉过敏方面更有效，但DRG和SDH的超微结构未发现变化。在DRG中检测到65个受推拿调节的差异表达基因（DEG），在SDH中检测到123个DEG。通过京都基因与基因组百科全书分析了推拿潜在的即时镇痛机制。DEG在DRG中富集于75条通路，在SDH中富集于107条通路。推拿的即时镇痛机制与钙信号通路、产热以及脂肪细胞中脂肪分解的调节有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/97b46ce3cc15/fnins-16-1007432-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/b2c7c097a9b6/fnins-16-1007432-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/a32d8b469559/fnins-16-1007432-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/869a5ba84c71/fnins-16-1007432-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/081f840e7408/fnins-16-1007432-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/1b1adc0f9240/fnins-16-1007432-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/97b46ce3cc15/fnins-16-1007432-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/b2c7c097a9b6/fnins-16-1007432-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/a32d8b469559/fnins-16-1007432-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/869a5ba84c71/fnins-16-1007432-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/081f840e7408/fnins-16-1007432-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/1b1adc0f9240/fnins-16-1007432-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a618/9577287/97b46ce3cc15/fnins-16-1007432-g006.jpg

相似文献

1
Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq.运用RNA测序技术探究单次推拿干预对轻微慢性缩窄性损伤大鼠即时镇痛作用的机制
Front Neurosci. 2022 Oct 4;16:1007432. doi: 10.3389/fnins.2022.1007432. eCollection 2022.
2
Exploring the Mechanism of Immediate Analgesia Induced by Tuina Intervention on Minor Chronic Constriction Injury in Rats Using LC-MS.基于液相色谱-质谱联用技术探究推拿干预对大鼠轻微慢性缩窄性损伤所致即时镇痛作用的机制
J Pain Res. 2024 Jan 23;17:321-334. doi: 10.2147/JPR.S438682. eCollection 2024.
3
Exploring the Analgesic Initiation Mechanism of Tuina in the Dorsal Root Ganglion of Minor CCI Rats via the TRPV1/TRPA1-cGMP Pathway.探讨经皮穴位电刺激通过 TRPV1/TRPA1-cGMP 通路对小CCI 大鼠背根神经节中推拿镇痛起始机制的影响。
Pain Res Manag. 2024 Jul 24;2024:2437396. doi: 10.1155/2024/2437396. eCollection 2024.
4
Chronic constriction injury-induced microRNA-146a-5p alleviates neuropathic pain through suppression of IRAK1/TRAF6 signaling pathway.慢性缩窄性损伤诱导的 microRNA-146a-5p 通过抑制 IRAK1/TRAF6 信号通路缓解神经病理性疼痛。
J Neuroinflammation. 2018 Jun 9;15(1):179. doi: 10.1186/s12974-018-1215-4.
5
Analyses of gene expression profiles in the rat dorsal horn of the spinal cord using RNA sequencing in chronic constriction injury rats.采用 RNA 测序分析慢性缩窄性损伤大鼠脊髓背角基因表达谱。
J Neuroinflammation. 2018 Sep 25;15(1):280. doi: 10.1186/s12974-018-1316-0.
6
Suppression of MyD88-dependent signaling alleviates neuropathic pain induced by peripheral nerve injury in the rat.抑制髓样分化因子88（MyD88）依赖性信号传导可减轻大鼠周围神经损伤所致的神经性疼痛。
J Neuroinflammation. 2017 Mar 31;14(1):70. doi: 10.1186/s12974-017-0822-9.
7
Characteristics of Pain Changes in Rats with Nerve Injury within 24 Hours after One-Time Tuina Intervention.一次性推拿干预后 24 小时内神经损伤大鼠疼痛变化的特征。
J Vis Exp. 2024 Jan 26(203). doi: 10.3791/65593.
8
Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon.推拿镇痛作用的可能机制可能涉及背根神经节轴突内的压电机械敏感通道。
J Tradit Chin Med. 2018 Dec;38(6):834-841.
9
Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain.推拿对背根节压迫性疼痛大鼠模型的镇痛作用
J Vis Exp. 2023 Jul 14(197). doi: 10.3791/65535.
10
High-voltage long-duration pulsed radiofrequency attenuates neuropathic pain in CCI rats by inhibiting Cav2.2 in spinal dorsal horn and dorsal root ganglion.高压长时程脉冲射频通过抑制脊髓背角和背根神经节中的 Cav2.2 来减轻 CCI 大鼠的神经病理性疼痛。
Brain Res. 2022 Jun 15;1785:147892. doi: 10.1016/j.brainres.2022.147892. Epub 2022 Mar 24.

引用本文的文献

1
Tuina Alleviates Neuropathic Pain in CCI Rats by Regulating the TRPV4-CaMKII Signaling Pathway in Dorsal Root Ganglion.推拿通过调节背根神经节中的TRPV4-CaMKII信号通路减轻CCI大鼠的神经性疼痛。
Pain Res Manag. 2025 Aug 6;2025:3697374. doi: 10.1155/prm/3697374. eCollection 2025.
2
Chinese massage therapy (Tuina) inhibits motor neuron apoptosis in rats with sciatic nerve injury by regulating the cPLA2 and RhoA/ROCK2 signaling pathways.中医按摩疗法（推拿）通过调节cPLA2和RhoA/ROCK2信号通路抑制坐骨神经损伤大鼠的运动神经元凋亡。
Front Neurol. 2025 Jul 30;16:1622602. doi: 10.3389/fneur.2025.1622602. eCollection 2025.
3

本文引用的文献

1
Anti-nociceptive effect of black seed oil on an animal model of chronic constriction injury.黑种草籽油对慢性压迫损伤动物模型的抗伤害感受作用。
Res Pharm Sci. 2022 Jul 14;17(4):383-391. doi: 10.4103/1735-5362.350239. eCollection 2022 Aug.
2
The Calcium Channel α2δ1 Subunit: Interactional Targets in Primary Sensory Neurons and Role in Neuropathic Pain.钙通道α2δ1亚基：初级感觉神经元中的相互作用靶点及在神经性疼痛中的作用
Front Cell Neurosci. 2021 Sep 30;15:699731. doi: 10.3389/fncel.2021.699731. eCollection 2021.
3
A Review on the Mechanism of Tuina Promoting the Recovery of Peripheral Nerve Injury.
Tuina promotes nerve myelin regeneration in SNI rats through Piezo1/YAP/TAZ pathway.
推拿通过Piezo1/YAP/TAZ通路促进坐骨神经损伤（SNI）大鼠的神经髓鞘再生。
J Orthop Surg Res. 2025 May 12;20(1):454. doi: 10.1186/s13018-025-05794-0.
4
A bibliometric analysis of the development trend of Tuina research: Based on the Web of Science (WOS) platform.基于科学网（WOS）平台的推拿研究发展趋势的文献计量分析
Medicine (Baltimore). 2025 Jan 17;104(3):e41070. doi: 10.1097/MD.0000000000041070.
5
Exploring the Analgesic Initiation Mechanism of Tuina in the Dorsal Root Ganglion of Minor CCI Rats via the TRPV1/TRPA1-cGMP Pathway.探讨经皮穴位电刺激通过 TRPV1/TRPA1-cGMP 通路对小CCI 大鼠背根神经节中推拿镇痛起始机制的影响。
Pain Res Manag. 2024 Jul 24;2024:2437396. doi: 10.1155/2024/2437396. eCollection 2024.
6
Research hotspots and trends of complementary and alternative therapy for neuropathic pain: A bibliometric analysis.神经性疼痛的补充和替代疗法研究热点与趋势：一项文献计量分析。
Medicine (Baltimore). 2024 May 3;103(18):e38054. doi: 10.1097/MD.0000000000038054.
7
Exploring the Mechanism of Immediate Analgesia Induced by Tuina Intervention on Minor Chronic Constriction Injury in Rats Using LC-MS.基于液相色谱-质谱联用技术探究推拿干预对大鼠轻微慢性缩窄性损伤所致即时镇痛作用的机制
J Pain Res. 2024 Jan 23;17:321-334. doi: 10.2147/JPR.S438682. eCollection 2024.
8
Resting-state functional magnetic resonance imaging reveals brain remodeling after Tuina therapy in neuropathic pain model.静息态功能磁共振成像揭示了推拿治疗神经病理性疼痛模型后大脑的重塑。
Front Mol Neurosci. 2023 Jul 12;16:1231374. doi: 10.3389/fnmol.2023.1231374. eCollection 2023.
9
Tuina for peripherally-induced neuropathic pain: A review of analgesic mechanism.推拿治疗外周性神经病理性疼痛：镇痛机制综述
Front Neurosci. 2022 Dec 22;16:1096734. doi: 10.3389/fnins.2022.1096734. eCollection 2022.
推拿促进周围神经损伤恢复机制的综述
Evid Based Complement Alternat Med. 2021 Jul 1;2021:6652099. doi: 10.1155/2021/6652099. eCollection 2021.
4
The effects of aromatherapy massage with lavender essential oil on neuropathic pain and quality of life in diabetic patients: A randomized clinical trial.薰衣草精油香薰按摩对糖尿病患者神经病理性疼痛及生活质量的影响：一项随机临床试验。
Complement Ther Clin Pract. 2021 Aug;44:101430. doi: 10.1016/j.ctcp.2021.101430. Epub 2021 Jun 18.
5
Silent cold-sensing neurons contribute to cold allodynia in neuropathic pain.沉默的冷觉神经元有助于神经性疼痛的冷感觉过敏。
Brain. 2021 Jul 28;144(6):1711-1726. doi: 10.1093/brain/awab086.
6
Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain.按摩对神经性疼痛大鼠TLR4信号通路的影响
Pain Res Manag. 2020 Dec 16;2020:8309745. doi: 10.1155/2020/8309745. eCollection 2020.
7
An Investigation into the Rehabilitative Mechanism of Tuina in the Treatment of Sciatic Nerve Injury.推拿治疗坐骨神经损伤的康复机制研究
Evid Based Complement Alternat Med. 2020 Jul 17;2020:5859298. doi: 10.1155/2020/5859298. eCollection 2020.
8
Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats.运用 RNA-Seq 技术探索三种方法和三穴针刺技术对坐骨神经损伤大鼠背根神经节修复机制的研究。
Pain Res Manag. 2020 Jun 7;2020:7531409. doi: 10.1155/2020/7531409. eCollection 2020.
9
A survey of chronic pain in China.中国慢性疼痛调查。
Libyan J Med. 2020 Dec;15(1):1730550. doi: 10.1080/19932820.2020.1730550.
10
Mild Mechanic Stimulate on Acupoints Regulation of CGRP-Positive Cells and Microglia Morphology in Spinal Cord of Sciatic Nerve Injured Rats.轻度机械刺激穴位对坐骨神经损伤大鼠脊髓中降钙素基因相关肽阳性细胞及小胶质细胞形态的调节作用
Front Integr Neurosci. 2019 Nov 14;13:58. doi: 10.3389/fnint.2019.00058. eCollection 2019.