[钴胺素衍生物对费氏丙酸杆菌谢氏亚种细胞中DNA合成的影响]

[Effect of cobalamin derivatives on DNA synthesis in cells of Propionibacterium freudenreichii subsp. shermanii].

作者信息

Iordan E P, Petukhova N I, Vorob'eva L I

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1987(1):5-10.

Abstract

The increase of DNA-synthesis rate (according incorporation [8-14C]adenine) in B12-deficient cells Propionibacterium shermanii as a result of different cobalamines adding into the cell suspension including metoxyethyladenile analog of adenozilcobalamin and some components of vitamin B12 molecule has been found. The DNA-synthesis rate in B12-deficient cells is nearly twice lower as compared with one in B12-normal cells. Considerable stimulative effect (80-100%) was provided with coenzyme forms of cobalamin. The data confirm the participation of vitamin B12 in DNA-synthesis in Propionibacterium cells.

摘要

已发现，由于向细胞悬液中添加了不同的钴胺素，包括腺苷钴胺的甲氧基乙基腺嘌呤类似物和维生素B12分子的一些成分，导致缺乏维生素B12的谢氏丙酸杆菌细胞中的DNA合成速率（根据[8-14C]腺嘌呤掺入量）增加。与维生素B12正常的细胞相比，缺乏维生素B12的细胞中的DNA合成速率几乎低两倍。钴胺素的辅酶形式具有相当大的刺激作用（80-100%）。这些数据证实了维生素B12参与谢氏丙酸杆菌细胞中的DNA合成。

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