Iordan E P, Novozhilova T Iu, Vorob'eva L I
Mikrobiologiia. 1983 Jul-Aug;52(4):591-6.
The content of DNA in Propionibacterium cells is nearly twice as low at vitamin B12 deficiency comparing with normal cells. The rate of labeled adenine incorporation into the DNA depends on vitamin B12 content in the cells. The addition of adenosylcobalamin and thymine to the medium makes DNA content rise in the cells of the B12-deficient culture. The addition of thymine to a suspension of B12-deficient cells accelerates the incorporation of labelled adenine into DNA. The authors discuss the phenomenon of bacterial "unbalanced division" and the control of replication by changing the level of DNA precursors.
与正常细胞相比，维生素B12缺乏时丙酸杆菌细胞中的DNA含量几乎低两倍。标记的腺嘌呤掺入DNA的速率取决于细胞中维生素B12的含量。向培养基中添加腺苷钴胺素和胸腺嘧啶可使维生素B12缺乏培养物的细胞中DNA含量增加。向维生素B12缺乏细胞的悬液中添加胸腺嘧啶可加速标记的腺嘌呤掺入DNA。作者讨论了细菌“不平衡分裂”现象以及通过改变DNA前体水平对复制的控制。
